Characterization of the In Situ Ecophysiology of Novel Phylotypes in Nutrient Removal Activated Sludge Treatment Plants

PloS One
Simon J McIlroyPer Halkjaer Nielsen

Abstract

An in depth understanding of the ecology of activated sludge nutrient removal wastewater treatment systems requires detailed knowledge of the community composition and metabolic activities of individual members. Recent 16S rRNA gene amplicon surveys of activated sludge wastewater treatment plants with nutrient removal indicate the presence of a core set of bacterial genera. These organisms are likely responsible for the bulk of nutrient transformations underpinning the functions of these plants. While the basic activities of some of these genera in situ are known, there is little to no information for the majority. This study applied microautoradiography coupled with fluorescence in situ hybridization (MAR-FISH) for the in situ characterization of selected genus-level-phylotypes for which limited physiological information is available. These included Sulfuritalea and A21b, both within the class Betaproteobacteria, as well as Kaga01, within sub-group 10 of the phylum Acidobacteria. While the Sulfuritalea spp. were observed to be metabolically versatile, the A21b and Kaga01 phylotypes appeared to be highly specialized.

References

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Nov 2, 2005·Methods in Enzymology·Jeppe Lund Nielsen, Per Halkjaer Nielsen
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Dec 16, 2011·The ISME Journal·Mads AlbertsenKåre Lehmann Nielsen
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Jan 17, 2013·Environmental Microbiology·Tomonori KindaichiPer Halkjaer Nielsen
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Nov 30, 2013·Nucleic Acids Research·James R ColeJames M Tiedje
Sep 3, 2014·Environmental Microbiology·Simon Jon McIlroyJeppe Lund Nielsen
Apr 1, 2015·The Journal of Clinical Pediatric Dentistry·A M Abdulla, A M Hegde
Jun 30, 2015·Database : the Journal of Biological Databases and Curation·Simon Jon McIlroyPer Halkjær Nielsen
Aug 12, 2015·The ISME Journal·Aaron M SaundersPer H Nielsen

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Citations

Jun 30, 2015·Database : the Journal of Biological Databases and Curation·Simon Jon McIlroyPer Halkjær Nielsen
Jun 1, 2017·Environmental Science and Pollution Research International·Jinlong LiShihai Deng
May 11, 2021·Frontiers in Microbiology·Jannie Munk KristensenPer Halkjaer Nielsen

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Methods Mentioned

BETA
amplicon-sequencing
mathFISH
amplicon sequencing
qFISH

Software Mentioned

MiDAS
ImageJ
Daime
mathFISH
ARB
qFISH
LOOPOUT

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