Characterization of the inhibitory effect of voriconazole on the fungicidal activity of amphotericin B against Candida albicans in an in vitro kinetic model

The Journal of Antimicrobial Chemotherapy
Anders LignellJan Sjölin

Abstract

The aim of the present investigation was to study and characterize the effect of voriconazole on the fungicidal activity of amphotericin B. Four strains of Candida albicans susceptible to voriconazole were exposed to voriconazole and amphotericin B, either alone, simultaneously or sequentially in an in vitro kinetic model. Bolus doses resulting in voriconazole and amphotericin B concentrations of 0.005-5 and 2.5 mg/L, respectively, were administered. Antifungal-containing RPMI 1640 was eliminated and replaced by a fresh medium using a peristaltic pump, with a flow rate adjusted to obtain the desired half-lives. With two drugs tested, a computer-controlled dosing pump compensated for differences in the elimination rates. Using static time-kill methodology, one C. albicans strain was exposed to 5 mg/L voriconazole for varying durations followed by 2.5 mg/L amphotericin B after three repeated washes of voriconazole. Voriconazole and amphotericin B treatment alone resulted in fungistatic and fungicidal activities, respectively. Simultaneous administration of voriconazole and amphotericin B resulted in fungicidal activity, whereas only fungistatic activity was observed when repeated doses of amphotericin B were administered sequenti...Continue Reading

References

Jan 11, 1992·Antimicrobial Agents and Chemotherapy·T L HodgesR C Moellering
Feb 1, 1990·Antimicrobial Agents and Chemotherapy·J BrajtburgG Medoff
Jan 1, 1983·Clinical Pharmacokinetics·T K Daneshmend, D W Warnock
Nov 1, 1980·Antimicrobial Agents and Chemotherapy·R D PearsonS W Chapman
Jan 27, 1994·The New England Journal of Medicine·J A Como, W E Dismukes
Nov 1, 1995·Antimicrobial Agents and Chemotherapy·M A GhannoumR S Criddle
Jan 9, 1999·Clinical Microbiology Reviews·D J SheehanC M Sibley
Feb 22, 2001·Antimicrobial Agents and Chemotherapy·D AndesR Conklin
Jan 25, 2002·The New England Journal of Medicine·Thomas J WalshUNKNOWN National Institute of Allergy and Infectious Diseases Mycoses Study Group
Jul 18, 2002·Antimicrobial Agents and Chemotherapy·L PurkinsD Kleinermans
May 15, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·John H RexUNKNOWN National Institute of Allergy and Infectious Diseases Mycoses Study Group
May 15, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Frank C Odds
Nov 18, 2003·British Journal of Clinical Pharmacology·Lynn PurkinsDon Nichols
Sep 18, 2004·The Journal of Antimicrobial Chemotherapy·Manuel Cuenca-Estrella
Mar 24, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A LignellJ Sjölin

❮ Previous
Next ❯

Citations

Oct 27, 2015·Environmental Science and Pollution Research International·Sofía Borrego, Ivette Perdomo
Oct 11, 2015·Environment International·Sotiris VardoulakisPaul Wilkinson
Oct 16, 2018·Indian Journal of Occupational and Environmental Medicine·Ruchika K JainMinal Trivedi
Nov 19, 2010·Expert Review of Hematology·Aneesh K Mehta, Amelia A Langston

❮ Previous
Next ❯

Related Concepts

Related Feeds

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.