Characterization of the interaction between nitrofurazone and human serum albumin by spectroscopic and molecular modeling methods

Molecular BioSystems
Fengyu DengYing Liu

Abstract

The interaction of nitrofurazone (NF) and human serum albumin (HSA) has been studied by fluorescence spectroscopy, FT-IR spectroscopy and molecular modeling methods. The results showed that the fluorescence of HSA was quenched by NF in a static quenching mechanism. Thermodynamic parameters revealed that hydrogen bonds and van der Waals force played the major role during the interaction. The calculated binding distance (r) indicated that the non-radioactive energy transfer came into being in the interaction between NF and HSA. HSA had a single class of binding site at Sudlow' site I in subdomain IIA for NF, which was verified by the displacement experiment. The molecular modeling study further confirmed the specific binding sites of NF on HSA, such as the interaction between N11 and N14 of NF with Lue 283 and Ser 287 predominately through hydrogen bonds. Three-dimensional fluorescence spectra indicated that the polarity around the tryptophan residues decreased and the conformation of HSA changed after adding NF. FT-IR spectra showed that NF could induce the polypeptides of HSA unfolding because it changed α-helix and β-sheet into β-turn and random structure of HSA.

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Citations

Oct 23, 2013·PloS One·Fernanda S Graciani, Valdecir F Ximenes
Dec 13, 2012·Luminescence : the Journal of Biological and Chemical Luminescence·Chengyu DongYing Liu
Apr 10, 2013·Biochimica Et Biophysica Acta·Shin-ichi Fujiwara, Takashi Amisaki
Feb 1, 2015·Journal of Pharmaceutical and Biomedical Analysis·Taoying Hu, Ying Liu
Dec 17, 2014·Colloids and Surfaces. B, Biointerfaces·Xiangrong Li, Su Wang

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