Characterization of the interactome of the porcine reproductive and respiratory syndrome virus glycoprotein-5

Archives of Virology
Maodong Zhang, Alexander N Zakhartchouk

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most important pathogens in the swine industry, causing reproductive failure in sows and respiratory disorders in piglets. Glycosylated protein 5 (GP5) is a major envelope protein of the virus. It is essential for virus particle assembly and involved in viral pathogenesis. In the present study, we identified the host cellular proteins that interact with GP5 by performing immunoprecipitation in MARC-145 cells infected by a recombinant PRRSV containing a FLAG-tag insertion in GP5. In total, 122 cellular proteins were identified by LC-MS/MS. Gene Ontology and KEGG databases were used to map these proteins to different cellular processes, locations and functions. Interestingly, 10.24% of identified cellular proteins were involved in the process of translation. Follow up experiments demonstrated that expression of GP5 in transfected cells led to inhibition of translation of reporter genes. Interaction between GP5 and ATP synthase subunit alpha (ATP5A) was further confirmed by co-immunoprecipitation suggesting a possible role of GP5 in regulation of ATP production in cells. These data contribute to a better understanding of GP5's role in viral pathogenesis and ...Continue Reading

References

Oct 16, 1999·Proceedings of the National Academy of Sciences of the United States of America·G van MarleE J Snijder
Jul 16, 2004·Journal of Virology·Peter L Delputte, Hans J Nauwynck
Oct 9, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ying FangAndrew E Firth
Feb 6, 2013·Archives of Virology·Liqian ZhuGuangzhi Tong
Aug 14, 2013·The Journal of General Virology·Eric J SnijderYing Fang
Dec 10, 2015·Annual Review of Animal Biosciences·Joan K LunneyGourapura J Renukaradhya

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Methods Mentioned

BETA
co-immunoprecipitation
bronchoalveolar
PCR
electrophoresis
Assay
Protein Assay
confocal microscopy
transfection
co-immunoprecipitation assay

Software Mentioned

Image J
GraphPad Prism
STRING
extractmsn
Sorcerer
Sequest

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