Characterization of the isoforms of MOVO zinc finger protein, a mouse homologue of Drosophila Ovo, as transcription factors

Gene
Sawako UnezakiSeiji Ito

Abstract

We previously described two isoforms (MOVO-A and -B) of a novel zinc finger protein MOVO, a mouse homologue of Drosophila Ovo protein. Here, we isolated cDNA encoding the third isoform MOVO-C, which had a transactivation domain and zinc finger domain, but lacked an N-terminal potential repression domain that was present in MOVO-A. Three isoform mRNAs were expressed highly in mouse testis and also in the ovary at lower levels. The structural analyses of the isolated Movo gene and mRNAs demonstrated that three different Movo transcripts were differentially processed to generate three isoforms. Major mRNA species encoded MOVO-B with a zinc finger domain alone, and minor mRNA species encoded MOVO-A (potential repressor) and MOVO-C (potential activator). To assign MOVO to a transcriptional factor, we characterized DNA-binding and transactivation properties. Random oligonucleotide selection, electrophoretic mobility shift assay and footprinting indicated that MOVO bound to the sequence, 5'-G(G/C/T)GGGGG-3'. These motifs were found in the 5'-flanking regions of Movo and other testis-specific genes. Nuclear proteins binding to this motif were detected in mouse testis, and the expression of MOVO mRNA was restricted in spermatocytes. The...Continue Reading

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Citations

Jan 12, 2011·Digestive Diseases and Sciences·Yoshiro ArakiA-Hon Kwon
Oct 16, 2007·Nitric Oxide : Biology and Chemistry·Katsuji TokuharaTadayoshi Okumura
Aug 8, 2007·Nitric Oxide : Biology and Chemistry·Masanori YamadaTadayoshi Okumura
Dec 28, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Kosuke MatsuiTadayoshi Okumura
Jun 19, 2007·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Sawako UnezakiSeiji Ito
Apr 17, 2008·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Małgorzata LenartowiczBarbara Bilińska
Dec 4, 2012·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Katsuji TokuharaA-Hon Kwon
Oct 13, 2011·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Takashi MatsuuraA-Hon Kwon
Jul 24, 2013·Biochemical and Biophysical Research Communications·Seiji HiraMasanori Mukai
Jan 7, 2017·Scientific Reports·Makoto HayashiSatoru Kobayashi
Nov 15, 2011·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·K MatsuiA-H Kwon
Aug 6, 2016·PloS One·Natalia IranoLucia Galvão de Albuquerque
Dec 5, 2019·Communications Biology·Shoichi NakamuraMasanori Mukai
Jan 12, 2020·Communications Biology·Shoichi NakamuraMasanori Mukai

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