Characterization of the kynurenine 3-monooxygenase gene corresponding to the white egg 1 mutant in the silkworm Bombyx mori

Molecular Genetics and Genomics : MGG
G-X QuanT Tamura

Abstract

Kynurenine 3-monooxygenase (KMO, EC 1.14.13.9), which catalyzes the oxidation of kynurenine to 3-hydroxykynurenine, is involved in the synthesis of ommochrome pigments in insects. A silkworm mutant, white egg 1 ( w-1), has been shown to be deficient in this enzyme activity. The mutant is characterized morphologically by its white eyes and the fact that the females lay white eggs. To analyze the relationship between the KMO gene and the mutation, we first determined the entire sequence of a full-length 2.0-kb cDNA and examined its expression pattern in the wild type. The cDNA sequence contains one ORF encoding a polypeptide of 456 amino acids, and transcripts were detected in the larval Malpighian tubules and the pupal ovaries, but not in other tissues. Southern analysis and nucleotide sequencing showed that the KMO gene is present in a single copy and consists of ten exons distributed over a 16-kb region. Comparison of the transcripts between the wild type and mutant silkworms showed that the wild type expressed a single transcript, whereas the mutant exhibited markedly reduced amounts of two transcripts with sizes of 2.0 kb and 1.8 kb. Nucleotide sequence analysis of these mutant transcripts indicated that sequences correspond...Continue Reading

Citations

Sep 10, 2004·Annual Review of Entomology·Marian R GoldsmithHiroaki Abe
Jul 22, 2015·Proceedings of the National Academy of Sciences of the United States of America·Takaaki DaimonTetsuro Shinoda
Dec 20, 2011·Insect Biochemistry and Molecular Biology·Isao KobayashiShuichiro Tomita
Oct 31, 2006·Journal of Insect Physiology·Qian HanJianyong Li
Sep 7, 2014·Journal of Bioscience and Bioengineering·Tsuyoshi NomuraKazuhito Fujiyama
Apr 26, 2003·Molecular Genetics and Genomics : MGG·Y KoikeT Shimada
Aug 12, 2005·Genome Génome / Conseil National De Recherches Canada·Yuji YasukochiHiroshi Fujii
Dec 28, 2018·Archives of Insect Biochemistry and Physiology·Colin S Brent, J Joe Hull
Feb 8, 2011·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Ken-ichiro TatematsuTakaaki Daimon
Dec 12, 2003·Genetics·Morikazu ImamuraToshiki Tamura

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