Characterization of the L-lactate dehydrogenase from Aggregatibacter actinomycetemcomitans.

PloS One
Stacie A Brown, Marvin Whiteley

Abstract

Aggregatibacter actinomycetemcomitans is a Gram-negative opportunistic pathogen and the proposed causative agent of localized aggressive periodontitis. A. actinomycetemcomitans is found exclusively in the mammalian oral cavity in the space between the gums and the teeth known as the gingival crevice. Many bacterial species reside in this environment where competition for carbon is high. A. actinomycetemcomitans utilizes a unique carbon resource partitioning system whereby the presence of L-lactate inhibits uptake of glucose, thus allowing preferential catabolism of L-lactate. Although the mechanism for this process is not fully elucidated, we previously demonstrated that high levels of intracellular pyruvate are critical for L-lactate preference. As the first step in L-lactate catabolism is conversion of L-lactate to pyruvate by lactate dehydrogenase, we proposed a model in which the A. actinomycetemcomitans L-lactate dehydrogenase, unlike homologous enzymes, is not feedback inhibited by pyruvate. This lack of feedback inhibition allows intracellular pyruvate to rise to levels sufficient to inhibit glucose uptake in other bacteria. In the present study, the A. actinomycetemcomitans L-lactate dehydrogenase was purified and shown...Continue Reading

References

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Jun 26, 2007·Journal of Bacteriology·Stacie A Brown, Marvin Whiteley

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Citations

Apr 13, 2011·PLoS Pathogens·Matthew M RamseyMarvin Whiteley
Sep 17, 2013·Journal of Cellular Biochemistry·Jamie A Meadows, Matthew J Wargo
Aug 15, 2015·Proteomics·Pedro Belda-FerreAlex Mira
Aug 27, 2013·Journal of Bacteriology·Peter JorthMarvin Whiteley
Dec 19, 2019·Periodontology 2000·Casey ChenJørgen Slots
Mar 11, 2021·Periodontology 2000·George Hajishengallis, Richard J Lamont
Jun 26, 2021·Microbial Ecology·Kenza DjemaiMaryam Tidjani Alou

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Datasets Mentioned

BETA
AA02749

Methods Mentioned

BETA
phosphotransferase
PCR
Protein Assay

Software Mentioned

BLASTp
SigmaPlot

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