Characterization of the lamin analogue NMCP2 in the monocot Allium cepa

Chromosoma
Malgorzata CiskaS Moreno Díaz de la Espina

Abstract

Nuclear lamina organization is similar in metazoan and plants though the latter lack orthologs of lamins, the main components of the metazoan lamina. Current evidence suggests that Nuclear Matrix Constituent Proteins (NMCPs) are the lamin analogues in plants as these proteins share several key features: higher-order secondary structure and domain layout, subnuclear distribution, and involvement in the regulation of nuclear shape and size, as well as in higher-order chromatin organization. Previously, we studied the NMCP family in flowering plants (angiosperms), in which it comprises two phylogenetic groups: NMCP1 and NMCP2. At present, in silico information about NMCP proteins in embryophytes is relatively advanced, though very few proteins, most of them of the NMCP1 type, have been extensively studied in vivo. We previously characterized the NCMP1 protein in the monocot Allium cepa. Here, we report the key features of a second protein of this species NMCP2, which presents a conserved sequence and domain layout. Immunofluorescence and immunoelectronmicroscopy evidence co-localization of endogenous AcNMCP2 and AcNMCP1 in the lamina, while Western blotting and immunoconfocal microscopy reveal a similar pattern of expression and d...Continue Reading

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Citations

Mar 5, 2019·Journal of Experimental Botany·Malgorzata CiskaSusana Moreno Díaz de la Espina
Nov 5, 2020·Molecular Biology of the Cell·Endia L BluntEric J Richards
Jul 16, 2021·Communicative & Integrative Biology·Pallavi Deolal, Krishnaveni Mishra

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Datasets Mentioned

BETA
LC150620

Methods Mentioned

BETA
scanning electron microscopy
transmission electron microscopy
immunoconfocal microscopy
confocal microscopy
PCR
Protein Assay

Software Mentioned

NucPred
Leica
confocal
MUSCLE
Leica Application Suite X
Quantity One
Clustal Omega
MEGA7 programme
MEGA7

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