Characterization of the Lassa virus matrix protein Z: electron microscopic study of virus-like particles and interaction with the nucleoprotein (NP)

Virus Research
Robert EichlerOliver Lenz


Lassa virus is the causative agent of a hemorrhagic fever endemic in west Africa. The RNA genome of Lassa virus encodes the glycoprotein precursor GP-C, a nucleoprotein (NP), the viral polymerase L and a small protein Z (11 kDa). Here, we analyze the role of Z protein for virus maturation. We have recently shown that expression of Z protein in the absence of other viral proteins is sufficient for the release of enveloped Z-containing particles. In this study, we examined particles secreted into the supernatant of a stably Z protein-expressing CHO cell line by electron microscopy. The observed Z-induced virus-like particles did not significantly differ in their morphology and size from Lassa virus particles. Mutation of two proline-rich domains within Z which are known to drastically reduce the release of virus-like particles, had no effect on the cellular localization of the protein nor on its membrane-association. Furthermore, we present evidence that Z interacts with the NP. We assume that Z recruits NP to cellular membranes where virus assembly takes place. We conclude from our data that Lassa virus Z protein plays an essential role in Lassa virus maturation.


Aug 1, 1991·Virology·J W Burns, M J Buchmeier
Mar 1, 1987·The Journal of Infectious Diseases·J B McCormickT C Tong
Apr 21, 2001·Virology·J TimminsW Weissenhorn
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·O LenzW Garten
Apr 23, 2002·Journal of Virology·Eric O Freed
Dec 24, 2002·Trends in Cell Biology·Owen PornillosWesley I Sundquist
Feb 1, 2003·Journal of Molecular Biology·Joanna TimminsWinfried Weissenhorn
Oct 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mar PerezJuan C de la Torre

❮ Previous
Next ❯


Nov 6, 2012·BMC Biotechnology·Cristina S BorioMario E Lozano
Mar 31, 2006·Journal of Virology·Shuzo UrataJiro Yasuda
Nov 7, 2006·Virology Journal·Thomas StreckerWolfgang Garten
Jul 3, 2007·Virology Journal·Shuzo UrataJiro Yasuda
May 29, 2008·Archives of Virology·Shuiyun LanYuying Liang
Jul 25, 2009·Journal of Virology·Sudhakar S AgnihothramJack H Nunberg
Jan 15, 2010·Journal of Virology·Katrin SchlieWolfgang Garten
Jan 29, 2010·Journal of Virology·Allison GrosethStephan Becker
Dec 17, 2010·Journal of Virology·Jesica M Levingston MacleodNora Lopez
May 25, 2012·Journal of Virology·W W Shanaka I RodrigoLuis Martínez-Sobrido
Dec 4, 2012·Viruses·Shuzo Urata, Jiro Yasuda
Dec 4, 2012·Viruses·Sarah Katharina FehlingThomas Strecker
Jan 25, 2013·Viruses·Juan C Zapata, Maria S Salvato
Feb 26, 2013·Viruses·Svenja WolffAllison Groseth
Apr 5, 2013·Archives of Virology·Frank LennartzWolfgang Garten
Oct 19, 2004·Critical Reviews in Clinical Laboratory Sciences·Stephan Günther, Oliver Lenz
May 23, 2012·Expert Opinion on Drug Discovery·Sheli R RadoshitzkySina Bavari
Feb 16, 2010·Biochemical and Biophysical Research Communications·C C GarcíaM S Salvato
May 4, 2015·Archives of Virology·Sheli R RadoshitzkyJuan Carlos de la Torre
Aug 27, 2016·Virologica Sinica·Wei WangGengfu Xiao
Jan 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kathryn M HastieErica Ollmann Saphire
Nov 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kathryn M HastieErica Ollmann Saphire
Jan 21, 2011·Journal of Virology·Olena ShtankoYoshihiro Kawaoka
Dec 18, 2013·Journal of Virology·Juan C ZapataMaria S Salvato
Feb 13, 2016·Journal of Virology·Bjoern Meyer, Hinh Ly

❮ Previous
Next ❯

Related Concepts

Related Feeds

Argentine Hemorrhagic Fever

Argentine hemorrhagic fever (AHF) is an endemo-epidemic disease caused by junín virus (JUNV), a member of the arenaviridae family. Discover the latest research on AHF here.