PMID: 11902937Mar 21, 2002Paper

Characterization of the major proteins of tubers of yam bean (Pachyrhizus ahipa)

Journal of Agricultural and Food Chemistry
Jane L Forsyth, P R Shewry

Abstract

Tubers of six accessions of ahipa (Pachyrhizus ahipa) contained between 0.77 and 1.34% nitrogen on a dry weight basis. This corresponds to 4.8 to 8.4% crude protein based on a nitrogen to protein conversion factor of 6.25; but detailed analysis of AC230 showed that although 93% of the total N was extracted with buffer containing 1.0 M NaCl, about a third of this was lost on dialysis. It was calculated, therefore, that salt-soluble proteins comprise about 60% of the total tuber nitrogen, with low-molecular-mass nitrogenous components comprising a further 30%. Electophoretic analysis of the salt-soluble proteins showed similar patterns of components in the six accessions, with none being present in amounts sufficiently high to suggest a role as storage proteins. Furthermore, light microscopy failed to show significant deposits of protein within the tuber cells. Five "major" protein bands, which together accounted for about 19% of the total salt-soluble protein fraction were purified and subjected to N-terminal amino acid sequencing. Comparison of these with sequences in protein databases revealed similarities to alpha-amylases, chitinases and chitin binding proteins, cysteine proteinases (including major components from P. erosus...Continue Reading

References

Feb 1, 1992·Plant Molecular Biology·D N CrowellR M Amasino
Apr 1, 1991·Plant Molecular Biology·T D SutliffR L Rodriguez
Nov 16, 1994·Biochimica Et Biophysica Acta·U M SoedjanaatmadjaJ J Beintema
Oct 6, 1997·Phytochemistry·A V GomesJ A Barnes
Aug 1, 1987·Molecular & General Genetics : MGG·D C BaulcombeM G Jarvis

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Citations

Nov 26, 2009·Evidence-based Complementary and Alternative Medicine : ECAM·Adelaida María Castro-SánchezJosé Manuel Quesada-Rubio
Feb 7, 2008·Physiologia Plantarum·Izabela S SantosValdirene M Gomes
Jul 6, 2013·Anais Da Academia Brasileira De Ciências·Rosilda M MussuryRosimeire Gassi

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