Characterization of the MCM homohexamer from the thermoacidophilic euryarchaeon Picrophilus torridus

Scientific Reports
Kasturi GoswamiSwati Saha

Abstract

The typical archaeal MCM exhibits helicase activity independently in vitro. This study characterizes MCM from the euryarchaeon Picrophilus torridus. While PtMCM hydrolyzes ATP in DNA-independent manner, it displays very poor ability to unwind DNA independently, and then too only under acidic conditions. The protein exists stably in complex with PtGINS in whole cell lysates, interacting directly with PtGINS under neutral and acidic conditions. GINS strongly activates MCM helicase activity, but only at low pH. In consonance with this, PtGINS activates PtMCM-mediated ATP hydrolysis only at low pH, with the amount of ATP hydrolyzed during the helicase reaction increasing more than fifty-fold in the presence of GINS. While the stimulation of MCM-mediated helicase activity by GINS has been reported in MCMs from P.furiosus, T.kodakarensis, and very recently, T.acidophilum, to the best of our knowledge, this is the first report of an MCM helicase demonstrating DNA unwinding activity only at such acidic pH, across all archaea and eukaryotes. PtGINS may induce/stabilize a conducive conformation of PtMCM under acidic conditions, favouring PtMCM-mediated DNA unwinding coupled to ATP hydrolysis. Our findings underscore the existence of dive...Continue Reading

References

Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·Z KelmanJ Hurwitz
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·J P ChongB Stillman
Jan 28, 2003·Nature Structural Biology·Ryan J FletcherXiaojiang S Chen
Jul 3, 2003·The Journal of Biological Chemistry·Jae-Ho ShinZvi Kelman
Aug 9, 2003·Nucleic Acids Research·Ian GraingeDale B Wigley
Sep 11, 2003·The Journal of Biological Chemistry·Mariarita De FeliceFrancesca M Pisani
Oct 21, 2003·EMBO Reports·Tillmann PapeSilvia Onesti
Jun 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·O FüttererW Liebl
Feb 1, 2005·Trends in Microbiology·Maria CiaramellaMosè Rossi
Aug 18, 2005·Nucleic Acids Research·Kira S MakarovaEugene V Koonin
Feb 18, 2006·EMBO Reports·Nina MarinsekStephen D Bell
May 9, 2006·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth R Jenkinson, James P J Chong
May 30, 2006·Journal of Structural Biology·Alessandro CostaSilvia Onesti
Oct 26, 2006·Nucleic Acids Research·Alessandro CostaSilvia Onesti
Nov 17, 2006·Nucleic Acids Research·Gyri Teien HauglandZvi Kelman
Mar 6, 2007·The Journal of Biological Chemistry·Biagio PucciFrancesca M Pisani
May 1, 2007·Trends in Cell Biology·Karim Labib, Agnieszka Gambus
Nov 8, 2007·The Journal of Biological Chemistry·Takehiro YoshimochiYoshizumi Ishino
Jan 11, 2008·Nucleic Acids Research·Nozomi SakakibaraZvi Kelman
Jan 23, 2008·Biochemical Society Transactions·Susan L Forsburg
Nov 13, 2008·The Journal of Biological Chemistry·Jae-Ho ShinZvi Kelman
Nov 13, 2008·Extremophiles : Life Under Extreme Conditions·Gyri Teien HauglandZvi Kelman
Dec 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Aaron S BrewsterXiaojiang S Chen
Jan 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth R BarryStephen D Bell
May 6, 2009·Molecular Microbiology·Nozomi SakakibaraZvi Kelman
Apr 14, 2010·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Masaki AkitaYoshizumi Ishino
May 6, 2010·Critical Reviews in Biochemistry and Molecular Biology·Aaron S Brewster, Xiaojiang S Chen
Dec 15, 2010·Archaea : an International Microbiological Journal·Nozomi SakakibaraZvi Kelman
Jun 10, 2011·Extremophiles : Life Under Extreme Conditions·Hiromi OginoYoshizumi Ishino
Aug 9, 2011·Nucleic Acids Research·Miao PanZvi Kelman
Aug 20, 2011·Current Opinion in Chemical Biology·Thomas R Beattie, Stephen D Bell
Nov 19, 2011·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Sonoko IshinoYoshizumi Ishino
Apr 25, 2013·Cold Spring Harbor Perspectives in Biology·Stephen P Bell, Jon M Kaguni
Jun 12, 2013·Annual Review of Biochemistry·Alessandro CostaJames M Berger
Aug 12, 2014·Extremophiles : Life Under Extreme Conditions·Hiromi OginoYoshizumi Ishino

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Methods Mentioned

BETA
gel filtration
immunoprecipitation
pulldown
electrophoresis
helicase assay
two hybrid
pull
PCR
Protein Assay

Software Mentioned

PtMCM
GraphPad Prism

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