PMID: 3534566Oct 1, 1986

Characterization of the mechanism of protein glycosylation and the structure of glycoconjugates in tissue culture trypomastigotes and intracellular amastigotes of Trypanosoma cruzi

Molecular and Biochemical Parasitology
P DoyleArmando J Parodi

Abstract

Trypomastigote cells of Trypanosoma cruzi incubated with [U-14C]glucose accumulated dolichol-P-P-linked Man7GlcNAc2 and Man9GlcNAc2. Evidence is presented indicating that both oligosaccharides were transferred to asparagine residues in proteins. On the other hand, intracellular amastigotes behaved as epimastigotes, i.e., only Man9GlcNAc2 accumulated and was transferred to proteins under similar incubation conditions. Intracellular amastigotes differed, therefore, from amastigotes obtained from an axenic culture, which behaved as trypomastigotes. A similar processing of protein-linked Man9GlcNAc2 and Man8GlcNAc2 occurred in epimastigotes and trypomastigotes but the structure of the main Man7GlcNAc2 isomer produced by demannosylation of the above mentioned oligosaccharides differed from that of the Man7GlcNAc2 transferred in trypomastigotes and amastigotes from axenic cultures. The infective trypomastigote stage of the parasite showed, therefore, an alteration in the mechanism of protein N-glycosylation when compared to the other stages, namely epimastigote (insect vector stage) and amastigote (mammalian intracellular stage). Complex-type, asparagine-bound oligosaccharides were found to be synthesized in both epimastigotes and tr...Continue Reading

References

Jun 1, 1985·Molecular and Biochemical Parasitology·J O PreviatoL Mendonça-Previato
Jun 1, 1985·Molecular and Biochemical Parasitology·R A MacinaA C Frasch
Mar 1, 1986·Molecular and Biochemical Parasitology·D H MendelzonArmando J Parodi
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Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·Armando J ParodiJuan J Cazzulo
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Citations

Nov 1, 1995·The Journal of Experimental Medicine·S KahnM Kahn
Sep 16, 2003·Molecular Biology of the Cell·Ianina ConteArmando J Parodi
Oct 22, 2005·Parasitology·V G DuschakE L D Isola
Jan 1, 1990·Journal of Psychoactive Drugs·R C Johnson, C T Nagoshi
Jan 1, 1993·Journal of Psychoactive Drugs·D Y Ja, B Aoki

Related Concepts

Paper Chromatography (Procedure)
Neoglycoproteins
Protein Glycosylation
Oligosaccharides
Trypanosoma cruzi

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