Characterization of the MEK5-ERK5 module in human neutrophils and its relationship to ERK1/ERK2 in the chemotactic response.

The Journal of Biological Chemistry
Charles S HiiAntonio Ferrante

Abstract

The role of the extracellular signal-regulated kinase (ERK) 1 and ERK2 in the neutrophil chemotactic response remains to be identified since a previously used specific inhibitor of MEK1 and MEK2, PD98059, that was used to provide evidence for a role of ERK1 and ERK2 in regulating chemotaxis, has recently been reported to also inhibit MEK5. This issue is made more critical by our present finding that human neutrophils express mitogen-activated protein (MAP) kinase/ERK kinase (MEK)5 and ERK5 (Big MAP kinase), and that their activities were stimulated by the bacterial tripeptide, formyl methionyl-leucyl-phenylalanine (fMLP). Dose response studies demonstrated a bell-shaped profile of fMLP-stimulated MEK5 and ERK5 activation, but this was left-shifted when compared with the profile of fMLP-stimulated chemotaxis. Kinetics studies demonstrated increases in kinase activity within 2 min, peaking at 3-5 min, and MEK5 activation was more persistent than that of ERK5. There were some similarities as well as differences in the pattern of activation between fMLP-stimulated ERK1 and ERK2, and MEK5-ERK5 activation. The up-regulation of MEK5-ERK5 activities was dependent on phosphatidylinositol 3-kinase. Studies with the recently described spe...Continue Reading

References

Dec 1, 1995·The Journal of Biological Chemistry·J M EnglishM H Cobb
Aug 15, 1995·Biochemical and Biophysical Research Communications·J D LeeJ Han
May 26, 1995·The Journal of Biological Chemistry·G ZhouJ E Dixon
Aug 1, 1994·The Journal of Clinical Investigation·G S WorthenG L Johnson
Jul 12, 1996·The Journal of Biological Chemistry·J AbeJ D Lee
Sep 22, 2000·The Biochemical Journal·S P DaviesP Cohen
Oct 24, 2000·The Journal of Biological Chemistry·I GotohE Nishida
Dec 21, 2000·American Journal of Physiology. Cell Physiology·J A LehmanJ Gómez-Cambronero
Apr 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mimi TakamiLilli Petruzzelli
Jul 18, 2002·Current Biology : CB·Sabine SuireLen Stephens
Sep 26, 2002·The Journal of Biological Chemistry·Gray W Pearson, Melanie H Cobb
Jan 18, 2003·Paediatric Respiratory Reviews·G Döring, D Worlitzsch
Jan 29, 2003·The Journal of Cell Biology·Supriya SrinivasanHenry R Bourne
Aug 13, 2003·The Journal of Biological Chemistry·Kazuhiro Nakamura, Gary L Johnson
Dec 19, 2003·The Journal of Biological Chemistry·Bing-E XuMelanie H Cobb

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Citations

Jul 30, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Wen-Fei ChiouBai-Luh Wei
Jun 24, 2011·PloS One·Marco A R VinoloRui Curi
Nov 29, 2008·European Journal of Pharmacology·María D CarrettaRafael A Burgos
Mar 25, 2008·Biochemical and Biophysical Research Communications·Lingli LiBradford C Berk
Aug 27, 2015·Science Signaling·Kevin WilhelmsenJudith Hellman
Dec 18, 2008·Journal of Cellular Physiology·Rajinder S SawhneyMichael G Brattain
May 30, 2006·Biochemical and Biophysical Research Communications·Shigeru KandaYasuyoshi Miyata
Dec 17, 2005·Molecular and Cellular Endocrinology·Girish Sharma, Marc L Goalstone
Feb 11, 2005·The Journal of Biological Chemistry·Zachary T Kneass, Richard B Marchase
Jun 1, 2006·The Journal of Biological Chemistry·Rebecca E SchweppeNatalie G Ahn
Oct 24, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Angela M FuselloAndrey S Shaw
Sep 26, 2015·Innate Immunity·Samira KhakpourJudith Hellman
Jun 30, 2016·Biotechnology and Applied Biochemistry·Wei JinSiwen Jiang
Jul 8, 2016·The Journal of Biological Chemistry·Daniele BologniniGraeme Milligan

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