Characterization of the metastatic phenotype of a panel of established osteosarcoma cells

Oncotarget
L RenC Khanna

Abstract

Osteosarcoma (OS) is the most common bone tumor in pediatric patients. Metastasis is a major cause of mortality and morbidity. The rarity of this disease coupled with the challenges of drug development for metastatic cancers have slowed the delivery of improvements in long-term outcomes for these patients. In this study, we collected 18 OS cell lines, confirmed their expression of bone markers and complex karyotypes, and characterized their in vivo tumorgenicity and metastatic potential. Since prior reports included conflicting descriptions of the metastatic and in vivo phenotypes of these models, there was a need for a comparative assessment of metastatic phenotypes using identical procedures in the hands of a single investigative group. We expect that this single characterization will accelerate the study of this metastatic cancer. Using these models we evaluated the expression of six previously reported metastasis-related OS genes. Ezrin was the only gene consistently differentially expressed in all the pairs of high/low metastatic OS cells. We then used a subtractive gene expression approach of the high and low human metastatic cells to identify novel genes that may be involved in OS metastasis. PHLDA1 (pleckstrin homology-...Continue Reading

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Methods Mentioned

BETA
surgical resection
chemical treatment
RNA-seq
RNA Seq
gene knock down
dissecting
fluorescence microscopy
transfection
Assay
FACS

Software Mentioned

Affymetrix GCOS
R
Cufflinks
Tophat
Openlab
Image J
affy
Bioconductor
simpleaffy

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