Characterization of the methanolic extract of hops using capillary electrophoresis-electrospray ionization-mass spectrometry

Electrophoresis
David Arráez-RománAlberto Fernández-Gutiérrez

Abstract

Hops are used almost exclusively for bitterness and flavor by brewers. We propose the first analytical application of CZE coupled to ESI-MS for the separation and structural elucidation of organic compounds in the methanolic extracts of hops, and different extraction procedures of the plant material have been carried out. The proposed method permits the identification of hop polyphenols (flavonoids glycosides and chalcones), bitter acids (alpha-acids and beta-acids), and their oxidation products. The optimization of CZE parameters (pH, concentration, and type of buffer) and ESI-MS parameters (nature and flow rate of the sheath liquid, nebulizer pressure, drying gas flow rate, temperature, and compound stability) have permitted the development of a rapid, simple, direct, and straightforward CZE-ESI-MS method for the identification of components of methanolic extracts from different hops used in the brewing process.

References

May 4, 2000·Journal of Agricultural and Food Chemistry·J F StevensM L Deinzer
Oct 11, 2001·Critical Reviews in Food Science and Nutrition·E Ibañez, A Cifuentes
Dec 5, 2003·Critical Reviews in Food Science and Nutrition·Javier SeñoransAlejandro Cifuentes
Jul 27, 2004·Electrophoresis·Javier Hernández-BorgesMatthias Pelzing
Feb 1, 2005·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Keiko SugaHiroshi Yamashita
Mar 12, 2005·Electrophoresis·Carolina SimóAlejandro Cifuentes

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Citations

Sep 28, 2007·Analytical and Bioanalytical Chemistry·D Arráez-RománA Fernández-Gutiérrez
Aug 20, 2010·Journal of Pharmaceutical and Biomedical Analysis·Elena Hurtado-FernándezAlberto Fernández-Gutiérrez
Mar 23, 2007·Journal of Chromatography. a·Maria Rowena N Monton, Tomoyoshi Soga
Nov 14, 2006·Journal of Chromatography. a·Javier Hernández-BorgesAlejandro Cifuentes
Apr 21, 2007·Journal of Separation Science·María Gómez-RomeroAlberto Fernández-Gutiérrez
Feb 28, 2007·Journal of Mass Spectrometry : JMS
Apr 11, 2009·Electrophoresis·Lidia M Ravelo-PérezMiguel Angel Rodríguez-Delgado
Oct 24, 2007·Electrophoresis·Jelena GorbatsovaMihkel Kaljurand
Sep 7, 2007·Electrophoresis·Christian W Klampfl
Nov 28, 2007·Electrophoresis·Virginia García-Cañas, Alejandro Cifuentes
Dec 8, 2011·Electrophoresis·Heide R RabanesJoselito P Quirino
Mar 17, 2015·Fitoterapia·Anna Katarzyna ŻołnierczykMirosław Anioł
Jul 1, 2013·Journal of Advanced Research·Mohamed A Farag, Ludger A Wessjohann
Oct 18, 2008·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·E-Hu LiuYong-Bo Peng
Dec 29, 2007·Electrophoresis·Andras GasparPhilippe Schmitt-Kopplin

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