Characterization of the microbial diversity in production waters of mesothermic and geothermic Tunisian oilfields

Journal of Basic Microbiology
Sami MnifMohamed Chamkha

Abstract

The microbial diversity of production waters of five Tunisian oilfields was investigated using Single Strand Conformation Polymorphism (SSCP) technique followed by cloning-sequencing. Dynamics of bacterial populations in production waters collected from four wellheads were also evaluated. For all production water samples collected, DNA from Archaea and Eucarya was not sufficiently abundant to permit detection rRNA genes from these groups by PCR-SSCP. In contrast, the bacterial rRNA genes were detected in all samples, except for samples from DOULEB12 and RAMOURA wells. SSCP profiles attested that two of the studied geothermic wells (ASHTART47 and ASHTART48) had shown a clear change over time, whereas a stable diversity was found with the mesophilic DOULEB well (DL3). PCR amplification of rRNA genes was unsuccessful with samples from DOULEB (DL12) at all three sampling time. The bacterial diversity present in production waters collected from pipelines of SERCINA and LITAYEM oilfields was high, while production waters collected from wellheads (ASHTART and DOULEB) exhibited lower diversity. The partial study of the biodiversity showed a dominance of uncultured bacteria and Pseudomonas genus (class of the Gammaproteobacteria) in thr...Continue Reading

References

May 15, 1981·Journal of Molecular Biology·J BrosiusH F Noller
May 13, 1999·International Journal of Systematic Bacteriology·N B HuuH Stan-Lotter
Jun 22, 1999·FEBS Letters·K O Stetter
Apr 18, 2000·Antonie van Leeuwenhoek·M MagotB K Patel
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Jonathan D Van HammeOwen P Ward
Jan 27, 2004·International Journal of Systematic and Evolutionary Microbiology·Elizabeth Miranda-TelloBernard Ollivier
Dec 8, 2005·FEMS Microbiology Ecology·Agnès GrabowskiChristian Jeanthon
Feb 3, 2007·International Journal of Systematic and Evolutionary Microbiology·Jun GuXiao-Lei Wu
Jun 7, 2007·International Journal of Systematic and Evolutionary Microbiology·Ya-Nan WangXiao-Lei Wu
Jun 26, 2007·Antonie van Leeuwenhoek·Håkon DahleNils-Kåre Birkeland
May 23, 2009·Anaerobe·Rory J MichellandLaurence Fortun-Lamothe
Oct 22, 2009·Applied Microbiology and Biotechnology·Aruliah RajasekarPattanathu K S M Rahman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Archaeogenetics

Recent advances in genomic sequencing has led to the discovery of new strains of Archaea and shed light on their evolutionary history. Discover the latest research on Archaeogenetics here.