Characterization of the microheterogeneity of transthyretin in plasma and urine using SELDI-TOF-MS immunoassay

Proteome Science
Florian J SchweigertJens Raila

Abstract

BACKGROUND: It has been shown that transthyretin (TTR) exists in different molecular variants. Besides point mutations associated with different diseases such as amyloidosis, other posttranslational modifications occur that might be of diagnostic interest. RESULTS: TTR levels as determined by ELISA in plasma and urine of healthy individuals were 489 +/- 155 microg/ml plasma and 46 +/- 24 ng/g creatinine, respectively. Average levels in urine of pregnant women were 45 +/- 65 microg/g creatinine. The molecular heterogeneity of TTR was analyzed using a high-throughput mass spectrometric immunoassay system. TTR was extracted from plasma or urine onto an antibody-coated (via protein A) affinity chip surface (PS20) using the surface-enhanced laser desorption/ionization (SELDI) technique. Subsequently samples were subjected to time-of-flight mass spectrometry (TOF-MS). In healthy individuals, TTR in plasma occurred rather consistently in two variants of 13732 +/- 12 and 13851 +/- 9 Da for the native and S-cysteinylated forms and at a smaller signal of 14043 +/- 17 Da for the S-glutathionylated form. In urine of pregnant women, various signals were observed with a dominant signal at 13736 +/- 10 Da and a varying number of smaller immun...Continue Reading

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Citations

Feb 8, 2006·Annals of Hematology·Eva M WeissingerBernd Hertenstein
Dec 20, 2005·European Archives of Psychiatry and Clinical Neuroscience·Jun LiLin He
May 4, 2007·Annals of Nutrition & Metabolism·Florian J Schweigert
Mar 13, 2013·Women's Health·Long NguyenDavid Fishman
Jan 13, 2006·Therapeutic Drug Monitoring·Yusheng ZhuSaeed A Jortani
Nov 18, 2009·Archives of Physiology and Biochemistry·Julia FröbelAkos Czibere
Nov 9, 2006·PLoS Medicine·Jeffrey T-J HuangSabine Bahn
May 4, 2016·Antioxidants & Redox Signaling·Pietro Ghezzi, Philippe Chan
Jun 1, 2006·Molecular & Cellular Proteomics : MCP·Dobrin NedelkovRandall W Nelson
Nov 13, 2014·Cellular and Molecular Life Sciences : CMLS·Florian T UngerKerstin A David
Oct 8, 2014·Proteomics. Clinical Applications·John F TimmsIan Jacobs
Nov 28, 2006·Rapid Communications in Mass Spectrometry : RCM·Sergey GontarevFlorian J Schweigert
Jun 1, 2007·Proteomics. Clinical Applications·Anne-Kristin FentzKerstin A David
Jul 1, 2008·Proteomics. Clinical Applications·Urban A KiernanRandall W Nelson
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Jul 26, 2005·Proteomics·Lynne ThadikkaranJean-Daniel Tissot
Nov 5, 2005·Molecular Nutrition & Food Research·Harshadrai M RawelFlorian J Schweigert
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Jun 1, 2007·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Florian J SchweigertKlaus Eulenberger

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Methods Mentioned

BETA
ELISA
affinity capture
electrophoresis
enzyme-linked immunoassay
chip

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