PMID: 2120189Oct 1, 1990Paper

Characterization of the minimum replicon of the broad-host-range plasmid pTF-FC2 and similarity between pTF-FC2 and the IncQ plasmids

Journal of Bacteriology
R A Dorrington, Douglas E Rawlings

Abstract

The nucleotide sequence of a 3,202-base-pair fragment which contained the minimum region required for replication of the broad-host-range plasmid, pTF-FC2, has been determined. At least five open reading frames and a region that affected the host range were identified. Proteins corresponding in size and location to four of the five open reading frames were produced in an in vitro transcription-translation system. The predicted amino acid sequences of two of the proteins were aligned with those of the RepA and RepC proteins of the broad-host-range IncQ plasmid RSF1010 and found to be 43 and 60% homologous, respectively. Despite this similarity, neither the RepA nor the RepC protein of the IncQ plasmid was able to complement mutations in the pTF-FC2 repA and repC genes. Although there was a considerable amount of DNA homology between pTF-FC2 and RSF1010 in the oriV region and the region coding for the RepA and RepC proteins, no other homology between the two plasmids at either the DNA or protein level could be detected.

References

Oct 24, 1986·Nucleic Acids Research·K Kim, R J Meyer
May 1, 1985·Applied and Environmental Microbiology·D E Rawlings, D R Woods
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·V HaringM Bagdasarian
Feb 1, 1974·Journal of Bacteriology·P GuerryS Falkow
Jul 10, 1981·Nucleic Acids Research·D Ish-Horowicz, J F Burke
May 1, 1982·Journal of Bacteriology·R MeyerM Brasch
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E ScherzingerM Bagdasarian
May 1, 1984·Journal of Bacteriology·D E RawlingsD R Woods
Jan 1, 1984·Annual Review of Microbiology·A P Harrison
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies

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Citations

Jun 8, 2002·FEMS Microbiology Letters·Eza KalyaevaVadim Nikiforov
Sep 1, 1996·World Journal of Microbiology & Biotechnology·A D Agate
Sep 1, 1993·Molecular Microbiology·J Rohrer, D E Rawlings
Dec 1, 2001·Microbiology and Molecular Biology Reviews : MMBR·D E Rawlings, E Tietze
Jun 30, 2000·Plasmid·T AparicioJ Perera
Nov 1, 2011·Plasmid·Wesley Loftie-Eaton, Douglas E Rawlings
Mar 1, 1994·Microbiological Reviews·D E Rawlings, T Kusano

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