Characterization of the mitochondrial genome of the MAX1 type of cytoplasmic male-sterile sunflower

BMC Plant Biology
Maksim S MakarenkoRenate Horn

Abstract

More than 70 cytoplasmic male sterility (CMS) types have been identified in Helianthus, but only for less than half of them, research of mitochondrial organization has been conducted. Moreover, complete mitochondrion sequences have only been published for two CMS sources - PET1 and PET2. It has been demonstrated that other sunflower CMS sources like MAX1, significantly differ from the PET1 and PET2 types. However, possible molecular causes for the CMS induction by MAX1 have not yet been proposed. In the present study, we have investigated structural changes in the mitochondrial genome of HA89 (MAX1) CMS sunflower line in comparison to the fertile mitochondrial genome. Eight significant major reorganization events have been determined in HA89 (MAX1) mtDNA: one 110 kb inverted region, four deletions of 439 bp, 978 bp, 3183 bp and 14,296 bp, respectively, and three insertions of 1999 bp, 5272 bp and 6583 bp. The rearrangements have led to functional changes in the mitochondrial genome of HA89 (MAX1) resulting in the complete elimination of orf777 and the appearance of new ORFs - orf306, orf480, orf645 and orf1287. Aligning the mtDNA of the CMS sources PET1 and PET2 with MAX1 we found some common reorganization features in their mi...Continue Reading

References

Feb 13, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·R. Horn
Jun 26, 2003·Nucleic Acids Research·A E KelE Wingender
May 8, 2004·The Plant Cell·Maureen R Hanson, Stéphane Bentolila
Jan 24, 2007·Bioinformatics·Arthur L DelcherSteven L Salzberg
Nov 27, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Young-Pyo LeeSoon-Kee Sung
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Jun 25, 2013·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Reetu TutejaRajeev K Varshney
Jun 25, 2013·Bioinformatics·Martin TriskaTatiana Tatarinova
Oct 1, 1994·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·V Hahn, W Friedt
Dec 10, 2013·Annual Review of Plant Biology·Letian Chen, Yao-Guang Liu
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Apr 16, 2014·Mitochondrion·Renate HornNoemi Colombo
Apr 29, 2014·Mitochondrion·Pascal Touzet, Etienne H Meyer
Feb 26, 2016·Plant Cell Reports·Abhishek BohraNarendra P Singh
Aug 30, 2017·Methods in Molecular Biology·Martin TriskaTatiana V Tatarinova
Feb 2, 2018·Frontiers in Plant Science·Aleksandra Dimitrijevic, Renate Horn
Mar 15, 2018·International Journal of Molecular Sciences·Antje Reddemann, Renate Horn

❮ Previous
Next ❯

Datasets Mentioned

BETA
MH704580.1

Methods Mentioned

BETA
PCR

Software Mentioned

bowtie
Match
SPAdes genome assembler
TMHMM
FastQC
Trimmomatic
cisExpress
glimmer
BLAST
OGDRAW

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.