Characterization of the mitochondrial porin from Drosophila melanogaster.

Biochimica Et Biophysica Acta
V De PintoF Palmieri

Abstract

Mitochondrial porin was isolated from the fruit fly Drosophila melanogaster at different developmental stages, starting from whole mitochondria. The porin from adults' mitochondria was fully characterized. The protein had a molecular mass of 31 kDa as judged from sodium dodecylsulfate electrophoretograms. It was very resistive against digestion with V8 proteinase of Staphylococcus aureus and a larger number of fragments were only obtained after digestion with papain. Drosophila porin showed little interaction with antibodies raised against mitochondrial porins from mammalia and Neurospora crassa, but a strong reactivity with antibodies raised against yeast porin. Reconstitution experiments with planar lipid bilayer membranes showed that the protein was able to form ion-permeable pores with a single-channel conductance of 0.41 nS in 0.1 M KCl. At low transmembrane voltages Drosophila porin had the properties of a general diffusion pore with an estimated effective diameter of about 1.7 nm and a small selectivity for anions over cations. Voltages larger than 20 to 30 mV resulted in a closure of the pore. The closed states of the pore were found to be cation-selective. The addition of a synthetic polyanion to the aqueous phase on o...Continue Reading

Citations

Feb 1, 1992·Journal of Bioenergetics and Biomembranes·V D De Pinto, F Palmieri
Apr 1, 1996·Journal of Bioenergetics and Biomembranes·W H Yu, M Forte
Aug 8, 2008·Journal of Bioenergetics and Biomembranes·Valeria SpecchiaVito De Pinto
Feb 27, 2010·FEBS Letters·Vito De PintoAlfons Lawen
Mar 30, 2010·Molecular Aspects of Medicine·Varda Shoshan-BarmatzNir Arbel
Dec 26, 2003·Biophysical Journal·Alexander G KomarovMarco Colombini
Jan 1, 1993·Critical Reviews in Biochemistry and Molecular Biology·M C Sorgato, O Moran
Jan 10, 2017·Frontiers in Oncology·Simona ReinaVito De Pinto
Apr 24, 2018·Frontiers in Chemistry·Andrea MagrìVito De Pinto
Jan 1, 1997·Progress in Biophysics and Molecular Biology·D R Laver, W G Peter
Jun 8, 2021·Frontiers in Physiology·Maria Carmela Di RosaVito De Pinto

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