Characterization of the modified Hodge test-positive isolates of Enterobacteriaceae in Taiwan

Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi
Kuei-Hsiang HungJiunn-Jong Wu

Abstract

The modified Hodge test is a phenotypic test to detect KPC-type carbapenemase producers among Enterobacteriaceae, as recommended by the Clinical Laboratory Standards Institute. However, false positive results were reported. In this study, we aimed to large-scale investigate the characterization of the modified Hodge test-positive isolates of Enterobacteriaceae collected between 2006 and 2010 in Taiwan. Fifty-six isolates, including 24 Enterobacter cloacae, 17 Escherichia coli, 10 Klebsiella pneumoniae, and 5 Citrobacter freundii, tested positive with the modified Hodge test. The in vitro activities of 10 antimicrobial agents were determined by the agar dilution method. Boronic acid combined-disk test was used to further confirm the KPC producers. Phenotype of ESBL, AmpC, class B carbapenemases, and profile of outer membrane proteins were investigated by the confirmatory test, boronic acid disk method, 2-mercaptopropionic acid double-disk method, and urea/sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), respectively. β-lactamase genes were examined by PCR and sequencing. These isolates were resistant to ceftazidime (100%), aztreonam (82.1%), ertapenem (64.3%), gentamicin (53.6%), ciprofloxacin (50%), levoflo...Continue Reading

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Citations

Apr 26, 2014·Future Microbiology·Shio-Shin Jean, Po-Ren Hsueh
Dec 3, 2014·International Journal of Antimicrobial Agents·Hung-Jen TangL Kristopher Siu
Aug 11, 2015·Journal of Chemotherapy·Abed Zahedi BialvaeiMehdi Yousefi
Apr 18, 2015·Indian Journal of Pathology & Microbiology·Leila AzimiElnaz Rastegar Lari
May 3, 2016·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Hung-Jen TangYin-Ching Chuang
Jan 12, 2017·Clinical Chemistry·Allison R McMullenCarey-Ann D Burnham
Dec 14, 2019·Antimicrobial Resistance and Infection Control·Alaa AbouelfetouhElsayed Aboulmagd

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