PMID: 9183013May 1, 1997Paper

Characterization of the molecular-chaperone function of the heat-shock-cognate-70-interacting protein

European Journal of Biochemistry
B D Bruce, J Churchich

Abstract

A histidine-tagged form of the recently discovered molecular chaperone, 70-kDa heat-shock cognate (Hsc70)-interacting protein (Hip), has been expressed in Escherichia coli and purified to near homogeneity. This protein remains soluble when expressed in E. coli. Several important properties of this chaperone have been investigated. HPLC size-exclusion chromatography indicates that the chaperone forms a tetramer similar to what has been reported for the native protein from rat liver cytosol. The recombinant form of Hip did not catalyze the hydrolysis of ATP and ATP analogs, although fluorescence measurements indicated that the chaperone recognizes anthraniloyl-dATP, anthraniloyl-ADP, and 2'-O-trinitrophenyl-ATP. The role of Hip as a molecular chaperone has been confirmed by its ability to strongly bind to the reduced, carboxymethylated form of alpha-lactalbumin. This interaction is specific for non-native domains since native alpha-lactalbumin fails to interact with Hip. Fluorescence-anisotropy measurements indicate that reduced, carboxymethylated lactalbumin binds Hip with a Kd of 5 microM. Although Hip appears to be able to bind nucleotides and non-native proteins, it is unable to facilitate the refolding of two denatured prote...Continue Reading

References

Nov 15, 1979·Analytical Biochemistry·P A LanzettaO A Candia
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A GragerovV Nikiforov
Jan 1, 1992·Annual Review of Biophysics and Biomolecular Structure·F U HartlW Neupert
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K LiberekM Zylicz
Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D R PallerosA L Fink
May 1, 1991·Trends in Biochemical Sciences·M Goebl, M Yanagida
Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T H NguyenD B Williams
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J FerréM Peferoen
Jan 20, 1995·The Journal of Biological Chemistry·Z LinE Eisenstein
Mar 29, 1993·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·S J Hardy, L L Randall
Sep 1, 1993·Analytical Biochemistry·J E Churchich
Dec 1, 1995·Molecular and Cellular Biology·D F SmithR A Rimerman

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Citations

Oct 20, 1998·Annals of Neurology·F R Sharp
Aug 24, 2001·Cell Biology International·P VerbekeS I Rattan
Apr 13, 1999·Trends in Neurosciences·F R SharpR A Swanson
Apr 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Carsten Lambert, Reinhild Prange
Dec 17, 2005·Journal of Zhejiang University. Science. B·Qing-hua DongJia-Yi Ding
Feb 3, 2009·Journal of Neurochemistry·Joanna L HowarthJames B Uney
Nov 15, 2000·The Journal of Biological Chemistry·E A NollenH H Kampinga
Apr 30, 1999·Physiological Reviews·A L Fink
Jan 13, 2000·Biochemistry·M VeltenM M Ladjimi

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