Characterization of the mouse bile salt export pump overexpressed in the baculovirus system

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Johannes NoeM V St-Pierre

Abstract

The bile salt export pump (Bsep), a member of the ATP-binding cassette superfamily of transporters, mediates the ATP-dependent canalicular secretion of bile salts. We have cloned and expressed the mouse Bsep (mBsep) protein in Sf9 insect cells, and characterized its transport and ATPase properties. Because its deduced amino acid sequence predicts multiple phosphorylation sites for protein kinase A, protein kinase C (PKC) and Ca(2+)-calmodulin dependent kinase II, we have also tested whether mBsep undergoes phosphorylation. MBsep transports both glycine and taurine conjugated bile salts. Sf9 cell membranes that express mBsep exhibit higher basal ATPase activity than control membranes, and this is further stimulated by bile salts and inhibited by vanadate. Taurochenodeoxycholate is transported with the highest affinity and is the most potent inducer of ATPase activity. Cyclosporin A, glibenclamide and rifamycin SV, all competitive inhibitors of Bsep transport, also reduced the bile salt-stimulated ATPase activity. MBsep exists as a phospho-protein when expressed in Sf9 cells and the immunoprecipitated mBsep complex is a substrate for the catalytic subunit of PKC. When mBsep and the alpha-isoform of mouse PKC are co-expressed in S...Continue Reading

Citations

Dec 4, 2004·Pflügers Archiv : European journal of physiology·Marco Arrese, Meenakshisundaram Ananthanarayanan
Oct 20, 2006·Pflügers Archiv : European journal of physiology·Bruno StiegerPeter J Meier
Apr 4, 2007·Pharmaceutical Research·Waddah A Alrefai, Ravinder K Gill
Nov 7, 2003·Current Gastroenterology Reports·Ronald Oude Elferink
Oct 30, 2001·Journal of Hepatology·B Stieger
Jun 22, 2002·Current Opinion in Genetics & Development·Laura N Bull
Apr 6, 2004·Clinics in Liver Disease·Gustav Paumgartner, Ulrich Beuers
Jun 3, 2006·Nature Clinical Practice. Gastroenterology & Hepatology·Ulrich Beuers
Jun 13, 2002·European Journal of Clinical Investigation·Helena GlasovaUlrich Beuers
Aug 29, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Gustav Paumgartner, Ulrich Beuers
Jan 26, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Tracy L MarionKim L R Brouwer
Oct 13, 2006·Current Opinion in Gastroenterology·Michael Trauner, James L Boyer
Jun 11, 2005·Journal of Gastroenterology and Hepatology·Matthew J HarrisIrwin M Arias
Jan 28, 2010·Pharmacological Reviews·Curtis D Klaassen, Lauren M Aleksunes
Mar 26, 2003·Gut·R Oude Elferink
Jun 5, 2002·Annual Review of Biochemistry·P Borst, R Oude Elferink
Feb 5, 2002·Annual Review of Physiology·Peter J Meier, B Stieger
Apr 11, 2009·World Journal of Gastroenterology : WJG·Maria-J Perez, Oscar Briz
Jul 16, 2009·Seminars in Immunopathology·Simon HohenesterUlrich Beuers
Nov 18, 2015·Expert Opinion on Therapeutic Targets·Ágnes Telbisz, László Homolya
Jul 17, 2012·Clinics and Research in Hepatology and Gastroenterology·Ralf KubitzDieter Häussinger
Feb 5, 2008·Clinics in Liver Disease·Gustav Paumgartner, Thomas Pusl
Apr 30, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Lifu WangJean-François Dufour
Mar 5, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M Sawkat Anwer
Feb 15, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Christopher M SchonhoffM Sawkat Anwer
Dec 23, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jane A ByrneRichard J Thompson
Nov 6, 2008·Biopharmaceutics & Drug Disposition·Hikaru YabuuchiIkumi Tamai
Aug 31, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Clemens SchafmayerJochen Hampe
Aug 18, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Lieke M van der VeldenStan F J van de Graaf
Jun 14, 2013·Liver International : Official Journal of the International Association for the Study of the Liver·Jan StindtRalf Kubitz
Apr 5, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M Sawkat Anwer
Nov 12, 2013·Biopharmaceutics & Drug Disposition·Lisl K M ShodaBrett A Howell
Jun 24, 2006·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Astrid BarthDieter Müller
May 21, 2013·Molecular Aspects of Medicine·Carol J Soroka, James L Boyer
Apr 18, 2002·Biochimica Et Biophysica Acta·Ronald Oude Elferink, Albert K Groen
Apr 10, 2016·Biopharmaceutics & Drug Disposition·Yaofeng ChengYurong Lai
Jan 9, 2003·Journal of Lipid Research·Maria E MendozaJose J G Marin
Dec 14, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Frank DombrowskiUlrich Beuers
Aug 1, 2008·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·A Kosters, S J Karpen
Jun 25, 2017·The Journal of Pharmacology and Experimental Therapeutics·Yaofeng ChengYurong Lai
Jun 13, 2009·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Emese KisPéter Krajcsi
Oct 4, 2011·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Sarah DawsonJ Gerald Kenna
May 20, 2005·The Journal of Pharmacology and Experimental Therapeutics·Masaru HiranoYuichi Sugiyama

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.