PMID: 11604102Oct 18, 2001Paper

Characterization of the mouse Dazap1 gene encoding an RNA-binding protein that interacts with infertility factors DAZ and DAZL

BMC Genomics
T DaiP H Yen

Abstract

DAZAP1 (DAZ Associated Protein 1) was originally identified by a yeast two-hybrid system through its interaction with a putative male infertility factor, DAZ (Deleted in Azoospermia). In vitro, DAZAP1 interacts with both the Y chromosome-encoded DAZ and an autosome-encoded DAZ-like protein, DAZL. DAZAP1 contains two RNA-binding domains (RBDs) and a proline-rich C-terminal portion, and is expressed most abundantly in the testis. To understand the biological function of DAZAP1 and the significance of its interaction with DAZ and DAZL, we isolated and characterized the mouse Dazap1 gene, and studied its expression and the subcellular localization of its protein product. The human and mouse genes have similar genomic structures and map to syntenic chromosomal regions. The mouse and human DAZAP1 proteins share 98% identity and their sequences are highly similar to the Xenopus orthologue Prrp, especially in the RBDs. Dazap1 is expressed throughout testis development. Western blot detects a single 45 kD DAZAP1 protein that is most abundant in the testis. Although a majority of DAZAP1 is present in the cytoplasmic fraction, they are not associated with polyribosomes. DAZAP1 is evolutionarily highly conserved. Its predominant expression...Continue Reading

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Citations

Jul 31, 2010·Journal of Biomedicine & Biotechnology·Paulo Navarro-CostaJoão Gonçalves
Aug 24, 2013·Nucleic Acids Research·Hsiang-Ying ChenPauline H Yen
Jul 20, 2006·The Biochemical Journal·Simon MortonSimon Rousseau
Mar 7, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Hye-Won SongMiles F Wilkinson
May 10, 2013·PloS One·Chi-Kai Yang, Pauline Yen
May 14, 2004·International Journal of Andrology·Pauline H Yen
Feb 20, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Matthew BrookNicola K Gray
Oct 8, 2005·Fertility and Sterility·Hsien-An PanPao-Lin Kuo
Feb 12, 2005·Reproductive Biomedicine Online·Nicola Reynolds, Howard J Cooke

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