PMID: 1202243Dec 1, 1975Paper

Characterization of the mRNA of influenza virus

Journal of Virology
S E GlassR D Barry

Abstract

The kinetics of the appearance of influenza mRNA, the distribution of mRNA between free and membrane-associated polyribosomes, its poly(A) content, and the extent to which the genome was transcribed into mRNA early in infection were determined. Polyribosomes were prepared from influenza virus-infected cells labeled for 30-min periods at various times after infection with [3H]uridine. Most of the 3H-labeled RNA extracted from these polyribosomes sedimented as a heterogeneous 8S to 20S peak in sucrose gradients, and it was largely complementary to virion RNA. By the following criteria, the complementary RNA had properties normally ascribed to mRNA: (i) it labeled rapidly with [3H]uridine; (ii) after glutaraldelyde treatment, it banded with polyribosomes in CsCl density gradients; and (iii) it contained poly(A). In chick cells at 37 C, virus mRNA was first detectable at 45 min postinfection and reached its maximal rate of appearance at 2 to 2.5 h postinfection. The free and membrane-bound polyribosomes of infected cells were separated and were found to contain the same classes of mRNA. There was no absolute segregation of mRNA sequences into either polyribosome class although each probably contained distinct ratios of the differen...Continue Reading

References

Apr 1, 1975·Virology·R J Avery, N J Dimmock
Apr 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·N L Chow, R W Simpson
Oct 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·W SiegertP H Hofschneider
Nov 1, 1974·Virology·P R Etkind, R M Krug
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Aug 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·Y Aloni, G Attardi

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Citations

Jan 1, 1980·Archives of Virology·G ContiG C Schito
Aug 28, 2012·Bioengineered·Junwei LiMingtao Zeng
May 29, 2015·Archives of Virology·Shashank TripathiSunil K Lal
Jan 1, 1982·Archives of Virology· Melnikov SYaY Z Ghendon
May 1, 1977·Journal of Virology·P R EtkindR M Krug
Oct 1, 1978·Virology·L A Caliguiri, H Gerstein
Jul 15, 1976·Virology·M B Richey, P Palese
Oct 15, 1979·Virology·T BarrettB W Mahy
Oct 1, 1976·Journal of Virology·D P NayakF O Wettstein

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