Characterization of the murine CTP:phosphocholine cytidylyltransferase beta gene promoter

Biochimica Et Biophysica Acta
Hebe MarcucciClaudia Banchio

Abstract

CTP:phosphocholine cytidylyltransferase (CCT) is a key regulatory enzyme in phosphatidylcholine (PtdCho) biosynthesis by the Kennedy pathway. In mammals, there are two genes that encode the enzyme isoforms that catalyze this reaction: Pcyt1a for CCTalpha and Pcyt1b for CCTbeta. In mouse tissues two different CCTbeta variants named CCTbeta2 and CCTbeta3 have been identified. Although little is known about Pcyt1b gene expression, recent data from cell lines propose a distinct role for CCTbeta2 in neuronal differentiation. Also, gonadal dysfunction in the CCTbeta2 knockout mouse suggests a role for this protein in ovary maturation and the maintenance of sperm production. This work defines and characterizes two alternative promoters that drive the expression of the two murine CCTbeta isoforms. The promoter activities were measured in Neuro-2a (mouse neuroblastoma), TM4 (mouse Sertoli) and C3H10T1/2 (mouse embryo fibroblast) cell lines. The transcriptional start points of each transcript and the promoter regions essential for the expression of each isoform were determined. Analysis of the CCTbeta2 promoter sequence suggested the transcription factor AP-1 as a potential regulator of CCTbeta2 expression in neuronal cells. However, CCT...Continue Reading

References

Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G B KalmarR B Cornell
May 25, 1982·Biochemistry·D R Voelker, E P Kennedy
Aug 11, 1995·The Journal of Biological Chemistry·Y Wang, C Kent
Jul 1, 1993·Frontiers in Neuroendocrinology·G E HoffmanJ G Verbalis
Jun 5, 1998·The Journal of Biological Chemistry·A LykidisS Jackowski
Apr 20, 1999·Biochemical and Biophysical Research Communications·J M Clement, C Kent
Dec 2, 1999·Experimental Cell Research·R Wisdom
Aug 10, 2001·Current Opinion in Structural Biology·C R Safinya
Dec 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Robert L StrausbergUNKNOWN Mammalian Gene Collection Program Team
Jul 5, 2003·Biochimica Et Biophysica Acta·Mohammad KarimSuzanne Jackowski
Aug 21, 2003·The Journal of Biological Chemistry·Jodi M CarterDennis E Vance
Mar 31, 2004·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Jean E Vance, Dennis E Vance
May 15, 2004·Molecular and Cellular Biology·Suzanne JackowskiMohammad A Karim
Apr 29, 2006·American Journal of Respiratory Cell and Molecular Biology·Diann M McCoyRama K Mallampalli

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Citations

Jun 8, 2010·The Journal of Biological Chemistry·Hebe MarcucciClaudia Banchio
Aug 6, 2011·IUBMB Life·Luciana PaolettiClaudia Banchio

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