Characterization of the murine hyaluronidase gene region reveals complex organization and cotranscription of Hyal1 with downstream genes, Fus2 and Hyal3

The Journal of Biological Chemistry
Tamara L ShuttleworthB Triggs-Raine

Abstract

Hyaluronidases are required for the breakdown of hyaluronan (HA), an abundant component of the extracellular matrix of vertebrate tissues. Multiple hyaluronidase genes have been identified, but the only clue to the function of their products has come from the identification of hyaluronidase 1 deficiency in a single patient with a mild clinical phenotype. As a first step in the generation of mice with hyaluronidase deficiency, we have used experimental and bioinformatic approaches to examine the organization of the mouse chromosome 9 region containing, in order, Hyal2, Hyal1, and Hyal3. This region was found to be complex, with Fus2 partially embedded in Hyal3, and Ifrd2 immediately downstream from Hyal3. The Hyal genes were all found to have four exons, and exons 2-4 exhibited the highest sequence conservation. Northern blot analysis demonstrated that the tissue expression profile for Hyal1 was similar in mice and humans, but a greater number of transcripts was detected in mouse tissues. Hyal3 was expressed more broadly in mice compared with humans and again exhibited additional transcripts. Reverse transcription-PCR demonstrated that some of the larger Hyal1 transcripts, seen on the Northern blot, were the result of cotranscri...Continue Reading

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Citations

Mar 9, 2006·Chemical Reviews·Robert Stern, Mark J Jedrzejas
Mar 18, 2008·Human Molecular Genetics·Dianna C MartinBarbara L Triggs-Raine
Jul 14, 2006·Neuroreport·Ahmed Al QteishatMark Slevin
Jul 13, 2012·American Journal of Physiology. Cell Physiology·Sarah CalveChristopher L Mendias
Sep 3, 2008·Matrix Biology : Journal of the International Society for Matrix Biology·Vasantha AtmuriBarbara Triggs-Raine
Mar 23, 2011·International Journal of Andrology·P A Martin-Deleon
Jul 28, 2004·Gene·Kesinee GatphayakBertram Brenig
May 23, 2006·Current Opinion in Structural Biology·Mihaela Zavolan, Erik van Nimwegen
Sep 19, 2014·Biology of Reproduction·Mark J ModelskiPatricia A Martin-DeLeon
Sep 1, 2015·World Journal of Biological Chemistry·Barbara Triggs-Raine, Marvin R Natowicz
Jun 27, 2002·The Journal of Biological Chemistry·Vinata B LokeshwarNaoko Iida
Aug 9, 2005·Pathologie-biologie·Robert Stern

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