Characterization of the Myeloid Cell Populations' Resident in the Porcine Palatine Tonsil

Frontiers in Immunology
Ferran SoldevilaFalko Steinbach

Abstract

The palatine tonsil is the portal of entry for food and air and is continuously subjected to environmental challenges, including pathogens, which use the tonsil and pharynx as a primary site of replication. In pigs, this includes the viruses causing porcine respiratory and reproductive syndrome, and classical and African swine fever; diseases that have impacted the pig production industry globally. Despite the importance of tonsils in host defense, little is known regarding the phenotype of the myeloid cells resident in the porcine tonsil. Here, we have characterized five myeloid cell populations that align to orthologous populations defined in other mammalian species: a CD4+ plasmacytoid dendritic cell (DC) defined by expression of the conserved markers E2.2 and IRF-7, a conventional dendritic cell (cDC1) population expressing CADM1highCD172alow and high levels of XCR1 able to activate allogeneic CD4 and CD8 T cells; a cDC2 population of CADM1dim cells expressing FLT3, IRF4, and CSF1R with an ability to activate allogeneic CD4 T cells; CD163+ macrophages (Mϴs) defined by high levels of endocytosis and responsiveness to LPS and finally a CD14+ population likely derived from the myelomonocytic lineage, which showed the highest l...Continue Reading

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Citations

Apr 16, 2019·Frontiers in Immunology·Efrain GuzmanMaria Montoya
May 19, 2019·Viruses·Giulia FranzoniAnnalisa Oggiano
Oct 22, 2019·European Journal of Immunology·Andrea CossarizzaArturo Zychlinsky
Aug 17, 2021·Frontiers in Immunology·Jesús HernándezEnric Mateu

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Methods Mentioned

BETA
flow cytometry
PCR
confocal microscopy
confocal

Software Mentioned

FACS Diva
Kaluza
MxPro QPCR
GraphPad Prism

Related Concepts