Characterization of the N-terminal repeat domain of Escherichia coli ClpA-A class I Clp/HSP100 ATPase

Protein Science : a Publication of the Protein Society
J H LoR T Sauer

Abstract

The ClpA, ClpB, and ClpC subfamilies of the Clp/HSP100 ATPases contain a conserved N-terminal region of approximately 150 residues that consists of two approximate sequence repeats. This sequence from the Escherichia coli ClpA enzyme is shown to encode an independent structural domain (the R domain) that is monomeric and approximately 40% alpha-helical. A ClpA fragment lacking the R domain showed ATP-dependent oligomerization, protein-stimulated ATPase activity, and the ability to complex with the ClpP peptidase and mediate degradation of peptide and protein substrates, including casein and ssrA-tagged proteins. Compared with the activities of the wild-type ClpA, however, those of the ClpA fragment missing the R domain were reduced. These results indicate that the R domain is not required for the basic recognition, unfolding, and translocation functions that allow ClpA-ClpP to degrade some protein substrates, but they suggest that it may play a role in modulating these activities.

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Citations

Mar 24, 2004·Journal of Structural Biology·Axel MogkBernd Bukau
Mar 24, 2004·Journal of Structural Biology·Di XiaMichael R Maurizi
Feb 11, 2003·Journal of Structural Biology·Andrei N Lupas, Kristin K Koretke
Dec 4, 2003·Biochemistry·Sabina KedzierskaMichal Zolkiewski
Jan 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Andrea KravatsGeorge Stan
Apr 23, 2002·Protein Science : a Publication of the Protein Society·Vekalet Tek, Michal Zolkiewski
Nov 27, 2015·Journal of Molecular Biology·Elizabeth A Sweeny, James Shorter
Nov 13, 2004·Protein Science : a Publication of the Protein Society·Naoki TanakaShigeru Kunugi
Apr 9, 2010·Molecular Microbiology·D A DouganK Zeth
May 30, 2006·Journal of Structural Biology·Sergej DjuranovicJörg Martin
Mar 8, 2003·Structure·Jingzhi Li, Bingdong Sha
Apr 5, 2002·Molecular Cell·David A DouganBernd Bukau
Jan 30, 2007·Journal of Molecular Biology·Eun Young ParkHyun Kyu Song
Mar 25, 2008·Journal of Molecular Biology·Susanne Cranz-MilevaEilika Weber-Ban
Nov 1, 2006·Trends in Biochemical Sciences·Tania A Baker, Robert T Sauer
Oct 11, 2003·FEBS Letters·Christine StrubAxel Mogk
Aug 26, 2003·The Journal of Biological Chemistry·Urszula A WojtyraWalid A Houry
Jul 30, 2020·Proceedings of the National Academy of Sciences of the United States of America·Yunlong LiAnil K Ojha
Sep 17, 2020·The Journal of Chemical Physics·Mohammad Sadegh AvestanGeorge Stan
Oct 4, 2003·The Journal of Biological Chemistry·Logan W DonaldsonWalid A Houry
Sep 28, 2002·The Journal of Biological Chemistry·Philipp BeinkerJochen Reinstein

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