Characterization of the nanostructure of complexes formed by a redox-active cationic lipid and DNA.

The Journal of Physical Chemistry. B
Claire L PizzeyYeshayahu Talmon

Abstract

We report characterization of the nanostructures of complexes formed between the redox-active lipid bis(n-ferrocenylundecyl)dimethylammonium bromide (BFDMA) and DNA using small-angle neutron scattering (SANS) and cryogenic transmission electron microscopy (cryo-TEM). A particular focus was directed to the influence of lipid oxidation state (where reduced BFDMA has a net charge of +1 and oxidized BFDMA has a charge of +3) on the nanostructures of the solution aggregates formed. Complexes were characterized over a range of charge ratios of reduced BFDMA to DNA (1.1:1, 2.75:1, and 4:1) in solutions of 1 mM Li2SO4. For these complexes, a single peak in the SANS data at 1.2 nm(-1) indicated that a nanostructure with a periodicity of 5.2 nm was present, similar to that observed with complexes of the classical lipids DODAB/DOPE and DNA (multilamellar spacing of 7.0 nm). The absence of additional Bragg peaks in all the SANS data indicated that the periodicity did not extend over large distances. Both inverse Fourier transform analysis and form factor fitting suggested formation of a multilamellar vesicle. These results were confirmed by cryo-TEM images in which multilamellar complexes with diameters between 50 and 150 nm were observed ...Continue Reading

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Citations

May 3, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Sharon GolanYeshayahu Talmon
Oct 4, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Burcu S AytarDavid M Lynn
Dec 14, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Arda AlkanFrederik R Wurm
Oct 26, 2016·Dalton Transactions : an International Journal of Inorganic Chemistry·Lalita B RaneAnupa A Kumbhar
Mar 13, 2015·Organic & Biomolecular Chemistry·Gururaja VulugundamSantanu Bhattacharya
Dec 22, 2009·Physical Chemistry Chemical Physics : PCCP·Conrad P CorbynMaria G Miguel
Feb 28, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Krishan KumarEmilio Aicart
Dec 18, 2008·Analytical Chemistry·Xiaoyang Liu, Nicholas L Abbott
Jan 5, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Sharon Golan, Yeshayahu Talmon

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