Characterization of the ndhC-psbG-ORF157/159 operon of maize plastid DNA and of the cyanobacterium Synechocystis sp. PCC6803

Molecular & General Genetics : MGG
K SteinmüllerL Bogorad

Abstract

The ndhC and ORF159 genes of the maize plastid DNA (ptDNA) were sequenced and maize ORF159 was used to screen a library of genomic DNA of the blue-green alga Synechocystis sp. PCC 6803. The cyanobacterial gene homologous to ORF159 (ORF157) was isolated and sequenced. In sequencing the region upstream of ORF157, reading frames with homology to the ndhC and psbG genes of maize ptDNA were identified. The ndhC and psbG genes overlap in the ptDNAs of maize, tobacco and Marchantia polymorpha, but are separated by a noncoding spacer in Synechocystis. Northern blot analysis showed that the ndhC, psbG and ORF157/159 genes are cotranscribed in maize and Synechocystis. The three genes occur in the same order in ptDNA of maize, tobacco, and M. polymorpha as in Synechocystis 6803. The amino acid sequences of the NDH-C, PSII-G and the ORF157/159 proteins deduced from the maize genes are 65%, 52% and 53% homologous to those of Synechocystis. However, the cyanobacterial and higher plant NDH-C protein sequences are only 23% homologous to the mitochondrial NDH-3 protein. Protein products of in vitro transcription/translation of the Synechocystis transcription unit had apparent molecular masses of 6 kDa (NDH-C), 25 kDa (PSII-G) and 22 kDa (ORF157...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
May 30, 1975·Science·L Bogorad
Feb 1, 1988·European Journal of Biochemistry·P Westhoff, R G Herrmann
May 11, 1987·Nucleic Acids Research·W E McLaughlin, I M Larrinua
Jan 1, 1985·Annual Review of Genetics·J D Palmer
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J Shine, L Dalgarno
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A ChomynR F Doolittle
Jan 1, 1980·Methods in Enzymology·A M Maxam, W Gilbert
Apr 1, 1981·Analytical Biochemistry·G DretzenP Chambon
Nov 15, 1983·Journal of Molecular Biology·A M FrischaufN Murray
May 25, 1984·Nucleic Acids Research·V Brendel, E N Trifonov
Sep 17, 1984·European Journal of Biochemistry·C L George, S J Ferguson
Apr 9, 1981·Nature·S AndersonI G Young
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·B MulliganL Bogorad
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J R BedbrookL Bogorad
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·Z SchwarzL Bogorad
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P Bennoun
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·G ZurawskiP R Whitfeld
May 1, 1983·Plant Molecular Biology·I M LarrinuaL Bogorad

❮ Previous
Next ❯

Citations

Oct 1, 1990·Plant Molecular Biology·Y TanakaK Yanagisawa
Nov 1, 1995·Photosynthesis Research·H MatsubaraY Fujita
Mar 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Maki Yukawa, Masahiro Sugiura
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T Ogawa
Jan 1, 1997·Microbial & Comparative Genomics·T Sicheritz-Pontén, S G Andersson
Jan 11, 1990·Nucleic Acids Research·A E PritchardD J Cummings
May 8, 1991·European Journal of Biochemistry·H WeissD Preis
Apr 1, 1993·Molecular & General Genetics : MGG·A MohamedC Jansson
Aug 1, 1992·Quarterly Reviews of Biophysics·J E Walker
Feb 8, 1993·Biochimica Et Biophysica Acta·T Yagi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.