PMID: 9171397Jun 1, 1997Paper

Characterization of the nitric oxide reductase-encoding region in Rhodobacter sphaeroides 2.4.3

Journal of Bacteriology
T B BartnikasJ P Shapleigh

Abstract

A gene cluster which includes genes required for the expression of nitric oxide reductase in Rhodobacter sphaeroides 2.4.3 has been isolated and characterized. Sequence analysis indicates that the two proximal genes in the cluster are the Nor structural genes. These two genes and four distal genes apparently constitute an operon. Mutational analysis indicates that the two structural genes, norC and norB, and the genes immediately downstream, norQ and norD, are required for expression of an active Nor complex. The remaining two genes, nnrT and nnrU, are required for expression of both Nir and Nor. The products of norCBQD have significant identity with products from other denitrifiers, whereas the predicted nnrT and nnrU gene products have no similarity with products corresponding to other sequences in the database. Mutational analysis and functional complementation studies indicate that the nnrT and nnrU genes can be expressed from an internal promoter. Deletion analysis of the regulatory region upstream of norC indicated that a sequence motif which has identity to a motif in the gene encoding nitrite reductase in strain 2.4.3 is critical for nor operon expression. Regulatory studies demonstrated that the first four genes, norCB...Continue Reading

References

Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jun 1, 1987·Microbiological Reviews·C R Woese
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·C T ChungR H Miller
Feb 25, 1987·Biochimica Et Biophysica Acta·J P ShapleighW J Payne
Sep 1, 1985·Journal of Bacteriology·J P Shapleigh, W J Payne
Jun 15, 1982·European Journal of Biochemistry·R B GennisB Ludwig
Dec 1, 1995·Journal of Bacteriology·H J WingS J Busby
Apr 4, 1995·Biochimica Et Biophysica Acta·H AraiT Kodama
Jan 1, 1994·Antonie van Leeuwenhoek·S Spiro
Jul 1, 1994·Bioscience, Biotechnology, and Biochemistry·H AraiT Kodama
Mar 14, 1994·FEBS Letters·M Saraste, J Castresana
Jan 1, 1993·Archives of Microbiology·W G Zumft
Dec 15, 1996·European Journal of Biochemistry·A P de BoerR J van Spanning

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Citations

Jan 8, 2013·Bioscience, Biotechnology, and Biochemistry·Hiroyuki AraiSamuel Kaplan
Jan 13, 2005·Canadian Journal of Microbiology·Yiu-Kwok Chan, Wayne A McCormick
Jan 27, 2011·Biochemical Society Transactions·James P Shapleigh
Jan 29, 2019·Microbiology Resource Announcements·Michael S Guzman, Arpita Bose
Jun 1, 2000·Bioscience, Biotechnology, and Biochemistry·M KukimotoS Horinouchi
Nov 23, 2005·The Journal of Biological Chemistry·Jamie SniderWalid A Houry
Apr 27, 2002·Applied and Environmental Microbiology·Dong Yun LeeJames P Shapleigh
Feb 24, 2006·Infection and Immunity·Séverine Loisel-MeyerVéronique Jubier-Maurin
Jun 6, 2003·Applied and Environmental Microbiology·Gesche Braker, James M Tiedje
Mar 7, 2006·Applied and Environmental Microbiology·Peter S ChoiJames P Shapleigh
Aug 9, 2005·Applied and Environmental Microbiology·Seung-Hun Baek, James P Shapleigh
Jun 7, 2005·Journal of Bacteriology·Peter S ChoiJames P Shapleigh
Jul 3, 2010·Microbiology·Angela Hartsock, James P Shapleigh
Mar 4, 2000·Applied and Environmental Microbiology·P W Coschigano
Sep 3, 2004·Journal of Bacteriology·Seung-Hun BaekJames P Shapleigh
Nov 19, 2020·FEMS Microbiology Ecology·Solène LecomteFeth El Zahar Haichar
Nov 5, 1999·Biochemical and Biophysical Research Communications·N R HayashiY Igarashi
May 13, 1999·Biochimica Et Biophysica Acta·N J WatmoughS Spiro
Oct 3, 2002·Biochimica Et Biophysica Acta·Laurent Philippot
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·S C BakerR J van Spanning
Dec 11, 1997·Journal of Bacteriology·J H Zeilstra-RyallsR G Kranz
Dec 31, 1997·Microbiology and Molecular Biology Reviews : MMBR·W G Zumft

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