Characterization of the novel antifungal protein PgAFP and the encoding gene of Penicillium chrysogenum

Peptides
Andrea Rodríguez-MartínMiguel A Asensio

Abstract

The strain RP42C from Penicillium chrysogenum produces a small protein PgAFP that inhibits the growth of some toxigenic molds. The molecular mass of the protein determined by electrospray ionization mass spectrometry (ESI-MS) was 6 494Da. PgAFP showed a cationic character with an estimated pI value of 9.22. Upon chemical and enzymatic treatments of PgAFP, no evidence for N- or O-glycosylations was obtained. Five partial sequences of PgAFP were obtained by Edman degradation and by ESI-MS/MS after trypsin and chymotrypsin digestions. Using degenerate primers from these peptide sequences, a segment of 70bp was amplified by PCR from pgafp gene. 5'- and 3'-ends of pgafp were obtained by RACE-PCR with gene-specific primers designed from the 70bp segment. The complete pgafp sequence of 404bp was obtained using primers designed from 5'- and 3'-ends. Comparison of genomic and cDNA sequences revealed a 279bp coding region interrupted by two introns of 63 and 62bp. The precursor of the antifungal protein consists of 92 amino acids and appears to be processed to the mature 58 amino acids PgAFP. The deduced amino acid sequence of the mature protein shares 79% identity to the antifungal protein Anafp from Aspergillus niger. PgAFP is a new pr...Continue Reading

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Citations

Jul 24, 2010·Applied Microbiology and Biotechnology·Andrea Rodríguez-MartínMiguel A Asensio
May 5, 2010·Journal of Industrial Microbiology & Biotechnology·Mohamed HajjiMoncef Nasri
Jul 2, 2014·Cell Biochemistry and Biophysics·Carlos PolancoMarili Leopold-Sordo
Oct 9, 2015·Applied Microbiology and Biotechnology·Josué DelgadoFélix Núñez
Jul 23, 2011·Journal of Basic Microbiology·Nikoletta HegedusIstván Pócsi
Jun 17, 2015·Applied Microbiology and Biotechnology·Josué DelgadoFélix Núñez
Feb 16, 2013·Fungal Biology Reviews·Nikoletta Hegedüs, Florentine Marx
Jul 12, 2011·Peptides·Laura KovácsLászló Galgóczy
Apr 16, 2015·International Journal of Food Microbiology·Josué DelgadoMiguel A Asensio
Jun 16, 2016·Protein & Cell·Chang-Wen WuHui Li
Jul 11, 2016·Applied Microbiology and Biotechnology·Josué DelgadoFélix Núñez
Dec 12, 2020·Journal of Pharmaceutical and Biomedical Analysis·Rekha Mol Kollakalnaduvil RaghavanElyas Kk
Feb 20, 2021·International Journal of Food Science·Noelia Zulema Palavecino PrpichMarcela Paola Castro
Mar 4, 2021·Food Microbiology·Pedro V Martínez-CulebrasPaloma Manzanares
Jul 3, 2021·Journal of Fungi·Mónica GandíaPaloma Manzanares

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