Characterization of the novel antifungal chitosanase PgChP and the encoding gene from Penicillium chrysogenum

Applied Microbiology and Biotechnology
Andrea Rodríguez-MartínMiguel A Asensio

Abstract

The protein PgChP is a new chitosanase produced by Penicillium chrysogenum AS51D that showed antifungal activity against toxigenic molds. Two isoforms were found by SDS-PAGE in the purified extract of PgChP. After enzymatic deglycosylation, only the smaller isoform was observed by SDS-PAGE. Identical amino acid sequences were obtained from the two isoforms. Analysis of the molecular mass by electrospray ionization-mass spectrometry revealed six major peaks from 30 to 31 kDa that are related to different levels of glycosylation. The pgchp gene has 1,146 bp including four introns and an open reading frame encoding a protein of 304 amino acids. The translated open reading frame has a predicted mass of 32 kDa, with the first 21 amino acids comprising a signal peptide. Two N glycosylation consensus sequences are present in the protein sequence. The deduced sequence showed high identity with fungal chitosanases. A high level of catalytic activity on chitosan was observed. PgChP is the first chitosanase described from P. chrysogenum. Given that enzymes produced by this mold species are granted generally recognized as safe status, PgChP could be used as a food preservative against toxigenic molds and to obtain chitosan oligomers for fo...Continue Reading

References

Oct 15, 1988·European Journal of Biochemistry·P WiesnerE Schweizer
Dec 20, 1995·Archives of Biochemistry and Biophysics·J LacadenaJ G Gavilanes
Sep 1, 1996·International Journal of Food Microbiology·F NúñezM A Asensio
Feb 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·O LeisF Hernández
Apr 25, 1997·Journal of Molecular Biology·C Burge, S Karlin
Jul 17, 1998·Current Opinion in Structural Biology·C B Burge, S Karlin
Oct 8, 1999·Biochemical and Biophysical Research Communications·D Gun LeeK S Hahm
Dec 15, 2000·Biotechnology and Applied Biochemistry·C Y Cheng, Y K Li
Nov 19, 2003·Biochemical and Biophysical Research Communications·A V DeepakBharathi P Salimath
Nov 19, 2003·International Journal of Food Microbiology·María J BenitoFélix Núñez
Jun 30, 2004·Journal of Molecular Biology·Jannick Dyrløv BendtsenSøren Brunak
Jul 17, 2004·Microbiology·David J Adams
Dec 7, 2005·The Journal of Biological Chemistry·Chih-Yu ChengYaw-Kuen Li
Dec 31, 2005·Journal of Bioscience and Bioengineering·Makoto ShimosakaMitsuo Okazaki
Jun 8, 2006·Journal of Agricultural and Food Chemistry·Caixin SuZengliang Yu
Jun 21, 2006·Proteomics·Willy MorelleJean-Claude Michalski
Aug 31, 2006·Applied Microbiology and Biotechnology·María J BenitoJuan J Córdoba
Dec 1, 2006·Biochimica Et Biophysica Acta·Hildegard Geyer, Rudolf Geyer
Sep 30, 2008·Nature Biotechnology·Marco A van den BergRoel A L Bovenberg
Oct 8, 2008·Nucleic Acids Research·Brandi L CantarelBernard Henrissat
Aug 18, 2009·International Journal of Food Microbiology·Raquel AcostaMiguel A Asensio
Jan 1, 1993·Bioscience, Biotechnology, and Biochemistry·M ShimosakaM Okazaki

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Citations

Dec 3, 2011·Applied Microbiology and Biotechnology·Lukas HartlVerena Seidl-Seiboth
Sep 19, 2012·Medical Mycology·Natacha B ProvostJ David Miller
Jul 11, 2016·Applied Microbiology and Biotechnology·Josué DelgadoFélix Núñez
Jan 19, 2019·World Journal of Microbiology & Biotechnology·Gleb E AktuganovVioletta R Safina

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