Characterization of the novel In1059 harbouring VIM gene cassette

Antimicrobial Resistance and Infection Control
Dongguo WangDongsheng Zhou

Abstract

VIM-type enzyme encodes the most widely acquired metallo-β-lactamases in Gram- negative bacteria. To obtain current epidemiological data for integrons from enterobacteriae in hospital, the study characterizes the genetic structure in In1059 by comparison with In846 integrons harbouring VIM gene and other class 1 integrons including In37, In62, In843 and In1021 with the aim of identifying the putative mechanisms involved integron mobilization and infer evolution of relevant integrons. Six of 69 recombinant plasmids from clinical strains were found to be class 1 integrons by digestion with BamHI, drug susceptibility testing, conjugation experiments, PCR amplification, integron cloning and sequencing, genome comparison, and detection of carbapenemase activity. The sequences of the six recombinant plasmids encoding In1021, In843, In846, In37, In62, and the novel In1059 integron had approximate lengths of ~4.8-, 4.1-, 5.1-, 5.3-, 5.3- and 6.6- kb, respectively. The genetic structures of these integrons were mapped and characterized, and the carbapenemase activities of their parental strains were assessed. Our results suggest that the six variable integron structures and regular variations that exist in the gene cassettes provide a p...Continue Reading

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Citations

Aug 16, 2019·Clinical Microbiology Reviews·Joaquim Ruiz

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Datasets Mentioned

BETA
KJ716225
DQ310703
KJ571202

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Inkscape
BLASTN

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