Characterization of the novel murine monoclonal anti-von Willebrand factor (vWf) antibody GUR76-23 which inhibits vWf interaction with alpha IIb beta 3 but not alpha v beta 3 integrin

Biochemical and Biophysical Research Communications
K YokoyamaY Ikeda

Abstract

von Willebrand factor (vWf) is known to interact with the two beta 3 integrins, alpha IIb beta 3 and alpha v beta 3, in an RGD-dependent manner. We characterized a novel murine monoclonal antibody to human vWf, GUR76-23, which recognized a site within the carboxy-terminal half of the molecule containing the RGD sequence. This antibody inhibited high shear-induced platelet aggregation and blocked adhesion of ADP plus epinephrine-stimulated platelets to vWf, indicating that it interferes with the interaction with alpha IIb beta 3. Unlike antibodies against the RGD site, however, the antibody was without effect on adhesion of cultured human umbilical vein endothelial cells to vWf, a phenomenon known to involve the interaction with alpha v beta 3. GUR76-23 binding was not displaced by anti-RGD antibodies. These results suggest that the adhesive interaction of vWf with these two beta 3 integrins may be differentially modulated by a site(s) other than the common RGD module.

References

Aug 30, 1992·Journal of Immunological Methods·M KatayamaY Ikeda
Apr 1, 1991·The Journal of Clinical Investigation·Y IkedaI Itagaki
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D A Cheresh
Jan 1, 1987·Annals of the New York Academy of Sciences·Y Kawai, R R Montgomery
Aug 15, 1984·Thrombosis Research·L Thorell, B Blombäck
Apr 22, 1994·Science·P C BrooksD A Cheresh
Apr 18, 1994·Annals of the New York Academy of Sciences·J J Sixma, P G de Groot

❮ Previous
Next ❯

Citations

Feb 25, 2000·Thrombosis Research·M Spadafora-FerreiraJ Kalil

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.