Characterization of the Olfactory Receptors Expressed in Human Spermatozoa

Frontiers in Molecular Biosciences
Caroline FlegelGunter Gisselmann

Abstract

The detection of external cues is fundamental for human spermatozoa to locate the oocyte in the female reproductive tract. This task requires a specific chemoreceptor repertoire that is expressed on the surface of human spermatozoa, which is not fully identified to date. Olfactory receptors (ORs) are candidate molecules and have been attributed to be involved in sperm chemotaxis and chemokinesis, indicating an important role in mammalian spermatozoa. An increasing importance has been suggested for spermatozoal RNA, which led us to investigate the expression of all 387 OR genes. This study provides the first comprehensive analysis of OR transcripts in human spermatozoa of several individuals by RNA-Seq. We detected 91 different transcripts in the spermatozoa samples that could be aligned to annotated OR genes. Using stranded mRNA-Seq, we detected a class of these putative OR transcripts in an antisense orientation, indicating a different function, rather than coding for a functional OR protein. Nevertheless, we were able to detect OR proteins in various compartments of human spermatozoa, indicating distinct functions in human sperm. A panel of various OR ligands induced Ca(2+) signals in human spermatozoa, which could be inhibit...Continue Reading

References

Jul 1, 1979·Journal of Reproduction and Fertility·D E Battalia, R Yanagimachi
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·A Cohen-DayagM Eisenbach
Dec 1, 1993·The Journal of Cell Biology·P VanderhaeghenM Parmentier
Mar 3, 1998·Journal of Hypertension. Supplement : Official Journal of the International Society of Hypertension·J P ClozelS I Ertel
May 3, 2000·The Journal of Biological Chemistry·G WennemuthD F Babcock
May 5, 2001·Genome Research·G GlusmanD Lancet
Jun 20, 2001·Anatomy and Embryology·K Steger
Sep 15, 2001·Nature·S Firestein
Oct 12, 2001·Trends in Endocrinology and Metabolism : TEM·N Dascal
Jan 22, 2002·Nature Neuroscience·Xinmin Zhang, Stuart Firestein
Sep 27, 2002·American Journal of Reproductive Immunology : AJRI·Andreas ZieglerBarbara Uchanska-Ziegler
Mar 15, 2003·The Journal of Biological Chemistry·Armin VolzAndreas Ziegler
May 14, 2004·Nature·G Charles OstermeierStephen A Krawetz
Dec 24, 2005·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Nanaho Fukuda, Kazushige Touhara
Apr 12, 2006·Nature Reviews. Molecular Cell Biology·Michael Eisenbach, Laura C Giojalas
May 24, 2006·BMC Genomics·Ester FeldmesserDoron Lancet
Jul 6, 2006·Journal of Cell Science·Eva M NeuhausHanns Hatt
Nov 15, 2007·Molecular BioSystems·Yutong SunXin-Yun Huang
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Nov 4, 2008·Nature·Eric T WangChristopher B Burge
Mar 6, 2009·Science Signaling·Harumi SaitoJoel D Mainland
Apr 25, 2009·The Journal of Biological Chemistry·Eva M NeuhausHanns Hatt
Jan 26, 2010·Reproductive Biology and Endocrinology : RB&E·Beena BhandariSatish K Gupta
Mar 18, 2011·Nature·Timo StrünkerU Benjamin Kaupp
Mar 18, 2011·Journal of Cellular Physiology·Pablo Martínez-LópezAlberto Darszon
Apr 2, 2011·The Journal of Biological Chemistry·Thomas VeitingerHanns Hatt
Oct 21, 2011·Physiological Reviews·Alberto DarszonClaudia L Treviño
Feb 23, 2012·The EMBO Journal·Christoph BrenkerTimo Strünker
Apr 21, 2012·Briefings in Bioinformatics·Helga ThorvaldsdóttirJill P Mesirov
Jul 19, 2012·PLoS Genetics·Kaylin A AdipietroHiroaki Matsunami
Feb 9, 2013·PloS One·Ivonne WallrabensteinGünter Gisselmann
Feb 14, 2013·PloS One·Caroline FlegelGünter Gisselmann
Mar 9, 2013·Nucleic Acids Research·Edward SendlerStephen A Krawetz
Nov 2, 2013·Current Genomics·James Dominic MillsMichael Janitz
May 14, 2014·EMBO Reports·Christian SchifferTimo Strünker

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Citations

Aug 31, 2016·Scientific Reports·Caroline FlegelGünter Gisselmann
Mar 14, 2018·Journal of Animal Science·Erin E ConnorGeorge E Liu
Dec 19, 2017·Frontiers in Physiology·Nikolina JovancevicHanns Hatt
Jun 14, 2018·Physiological Reviews·Désirée Maßberg, Hanns Hatt
Dec 24, 2018·Nucleic Acids Research·Molly S EstillStephen A Krawetz
Sep 22, 2017·Frontiers in Molecular Biosciences·Fabrizio FierroPaolo Carloni
Aug 20, 2020·Proceedings. Biological Sciences·Annalaura JokiniemiJukka Kekäläinen
Jul 28, 2018·Proceedings. Biological Sciences·Jukka Kekäläinen, Jonathan P Evans
Dec 14, 2018·Frontiers in Physiology·Nicholas M DalesioDan E Berkowitz
Jun 19, 2019·Proceedings of the National Academy of Sciences of the United States of America·David C RinkerJohn G Gibbons
Apr 16, 2020·International Journal of Molecular Sciences·Michaela FrolikovaKaterina Komrskova
Sep 10, 2020·International Journal of Molecular Sciences·Ahmed M El-ShehawiMona M Elseehy
Feb 9, 2017·Frontiers in Cellular Neuroscience·Nikolina JovancevicLian Gelis
May 14, 2017·Proceedings of the National Academy of Sciences of the United States of America·Ross D HoehnSabre Kais
May 2, 2019·American Journal of Physiology. Renal Physiology·Victoria L Halperin KuhnsJennifer L Pluznick
Mar 23, 2019·International Journal of Molecular Sciences·Antonella Di PizioDietmar Krautwurst
Sep 20, 2018·Nature Communications·Jérémy ChéretRalf Paus
Oct 22, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Romain DurouxEstelle Loing
Oct 25, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jessie HuangSteven S An
Feb 20, 2021·Critical Reviews in Food Science and Nutrition·Rifat Nowshin RakaJianming Jin
Jul 16, 2021·Reproductive Medicine and Biology·Hiromitsu Tanaka, Akira Tsujimura
May 25, 2021·American Journal of Physiology. Renal Physiology·Brian G PollMark A Knepper
Apr 13, 2019·Biochemistry·Emily A YasiPamela Peralta-Yahya
Aug 14, 2021·Frontiers in Physiology·Daniel WeidingerHanns Hatt

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Datasets Mentioned

BETA
E-MTAB-1733
GSE30611
GSE12946

Methods Mentioned

BETA
RNA-Seq
PCR
transfections
Illumina sequencing
imaging technique

Software Mentioned

Cufflinks
CatSper
Human Bodymap Atlas
TopHat
Integrative Genomic Viewer ( IGV )

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