Characterization of the Oregon isolate of Neospora hughesi from a horse

The Journal of Parasitology
J P DubeyM C Jenkins

Abstract

Neospora hughesi was isolated in cell cultures inoculated with homogenate of spinal cord from a horse in Oregon. Tachyzoites of this Oregon isolate of N. hughesi were maintained continuously by cell culture passage and tachyzoites were infective to immunosuppressed mice. Gamma interferon gene knockout (KO) mice injected with tachyzoites developed fatal myocarditis and numerous tachyzoites were seen in lesions. Gerbils (Meriones unguiculatus) inoculated with tachyzoites developed antibodies (> or = 1:500) as indicated by the Neospora caninum agglutination test but did not develop clinical signs, and Neospora organisms were not demonstrable in their tissues. Tissue cysts were not found in gerbils, nude mice, KO mice, immunosuppressed outbred Swiss Webster mice, or BALB/c mice injected with the Oregon isolate of N. hughesi. Ultrastructurally, tachyzoites of the Oregon isolate from the myocardium of infected KO mice and from cell culture were similar to N. caninum tachyzoites. Western blot analysis using NcSAG1 and NcSRS2 polyclonal and monoclonal antibodies and characterization of the internal transcribed spacer 1 sequences from the equine isolates and different isolates of N. caninum from dogs and cattle indicated that the Oregon...Continue Reading

References

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Citations

Feb 28, 2001·International Journal for Parasitology·C P WalshD S Lindsay
Jan 29, 2002·International Journal for Parasitology·Siobhan P EllisonJohn B Dame
Jun 22, 2002·International Journal for Parasitology·J P DubeyD S Lindsay
Nov 9, 2002·Veterinary Parasitology·Jan R SlapetaBretislav Koudela
Feb 13, 2003·Veterinary Parasitology·C HyunA E Marsh
Dec 3, 2014·The Veterinary Clinics of North America. Equine Practice·Daniel K HoweStephen M Reed
Apr 13, 2007·Clinical Microbiology Reviews·J P DubeyL M Ortega-Mora
Feb 18, 2005·The Journal of Parasitology·Paulo C DuarteIan A Gardner
Aug 18, 2009·Molecular and Cellular Probes·Sarwat Al-QassabJohn Ellis
May 10, 2008·Journal of Veterinary Internal Medicine·P S MorleyB A Wagner
Jul 21, 2004·Veterinary Parasitology·C M OkeomaL Gillespie
Apr 18, 2006·Trends in Parasitology·Luís F P Gondim
Oct 30, 2010·Veterinary Parasitology·Rodrigo Martins SoaresLeonardo José Richtzenhain
Jun 26, 2012·Veterinary Parasitology·Pengfei HeXichen Zhang
Mar 24, 2016·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Michelle Klein SercundesRodrigo Martins Soares
Jan 30, 2014·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Anderson Barbosa de MouraSilvério Bunn
Apr 27, 2007·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Carrie J FinnoNicola Pusterla
Jun 28, 2001·The Journal of Parasitology·W BassoJ P Dubey
Apr 2, 2003·The Korean Journal of Parasitology·J P Dubey, John Dubey
Sep 9, 2005·Clinical and Diagnostic Laboratory Immunology·Jessica S HoaneDaniel K Howe
Mar 28, 2013·Journal of Chemical Biology·Samuel FurseDavid A Gray
Jun 11, 2020·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Marwan Abu-HalawehRaida Khalil
Mar 27, 2001·Equine Veterinary Journal·D S Lindsay

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Datasets Mentioned

BETA
AF249971

Methods Mentioned

BETA
gene knockout
light microscopy
transmission electron microscopy
PCRs
electrophoresis

Software Mentioned

UWGCG

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