PMID: 9006050Feb 1, 1997Paper

Characterization of the p-toluenesulfonate operon tsaMBCD and tsaR in Comamonas testosteroni T-2

Journal of Bacteriology
F JunkerA M Cook

Abstract

Comamonas testosteroni T-2 uses a standard, if seldom examined, attack on an aromatic compound and oxygenates the side chain of p-toluenesulfonate (TS) (or p-toluenecarboxylate) to p-sulfobenzoate (or terephthalate) prior to complete oxidation. The expression of the first three catabolic enzymes in the pathway, the TS methyl-monooxygenase system (comprising reductase B and oxygenase M; TsaMB), p-sulfobenzyl alcohol dehydrogenase (TsaC), and p-sulfobenzaldehyde dehydrogenase (TsaD), is coregulated as regulatory unit R1 (H. R. Schlafli Oppenberg, G. Chen, T. Leisinger, and A. M. Cook, Microbiology [Reading] 141:1891-1899, 1995). The components of the oxygenase system were repurified, and the N-terminal amino acid sequences were confirmed and extended. An internal sequence of TsaM was obtained, and the identity of the [2Fe-2S] Rieske center was confirmed by electron paramagnetic resonance spectroscopy. We purified both dehydrogenases (TsaC and TsaD) and determined their molecular weights and N-terminal amino acid sequences. Oligonucleotides derived from the partial sequences of TsaM were used to identify cloned DNA from strain T-2, and about 6 kb of contiguous cloned DNA was sequenced. Regulatory unit R1 was presumed to represent ...Continue Reading

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Citations

Jun 1, 2017·Nature Communications·Zi Wei Luo, Sang Yup Lee
Sep 30, 2017·FEMS Yeast Research·Vasil D'Ambrosio, Michael K Jensen
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·A TanakaK Okada
Feb 17, 1999·FEMS Microbiology Reviews·A M CookF Junker
May 19, 2019·Genes·Manuel SalvadorJose I Jimenez
May 19, 2006·Journal of Bacteriology·Miguel A ProvidentiIain B Lambert
Feb 15, 2000·Journal of Bacteriology·B MorawskiL N Ornston
Jun 1, 1997·Applied and Environmental Microbiology·F Junker, A M Cook
Dec 16, 1998·Journal of Bacteriology·H K Chang, G J Zylstra

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