Characterization of the PcCdc42 small G protein from Pneumocystis carinii, which interacts with the PcSte20 life cycle regulatory kinase.

American Journal of Physiology. Lung Cellular and Molecular Physiology
Bryan J KrajicekAndrew H Limper

Abstract

Pneumocystis carinii (Pc) causes severe pneumonia in immunocompromised hosts. The binding of Pc trophic forms to alveolar epithelial cells is a central feature of infection, inducing the expression and activation of PcSte20, a gene participating in mating, proliferation, and pseudohyphal growth. In related fungi, Ste20 proteins are generally activated by immediate upstream small G proteins of the Cdc42-like family. PcCdc42 has not been previously described in Pneumocystis. To address the potential role of such a G protein in Pneumocystis, PcCdc42 was cloned from a Pc cDNA library. Using the full-length 576-bp PcCdc42 cDNA sequence, a CHEF blot of genomic DNA yielded a single band, providing evidence that this gene is present as a single copy within the genome. The total length of PcCdc42 cDNA was 576 bp with an estimated molecular mass of approximately 38 kDa. BLASTP analysis demonstrated greater than 80% homology with other fungal Cdc42p proteins. Northern analysis indicated equal mRNA expression in both cystic and trophic life forms. Heterologous expression of PcCdc42 in Saccharomyces cerevisiae (Sc) demonstrated that PcCdc42p was able to restore growth in an ScCdc42Delta yeast strain. Additional assays with purified PcCdc42 ...Continue Reading

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Citations

Jul 29, 2015·Medical Microbiology and Immunology·Theodore J Kottom, Andrew H Limper
Jun 14, 2015·FEMS Yeast Research·Joseph H SkalskiAndrew H Limper
Dec 1, 2010·FEMS Yeast Research·Magali ChabéEduardo Dei-Cas
Jun 27, 2013·The Journal of Biological Chemistry·Theodore J Kottom, Andrew H Limper
Mar 17, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Theodore J KottomAndrew H Limper
Apr 29, 2015·Infection and Immunity·Theodore J KottomAndrew H Limper
Jan 15, 2015·MBio·João M G C F AlmeidaPhilippe M Hauser
Jun 18, 2020·Cellular Microbiology·Theodore J KottomAndrew H Limper
May 5, 2020·The Journal of Infectious Diseases·Theodore J KottomAndrew H Limper
Sep 9, 2017·American Journal of Respiratory Cell and Molecular Biology·Theodore J KottomAndrew H Limper

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