PMID: 2499869May 25, 1989Paper

Characterization of the phage phi 29 protein p5 as a single-stranded DNA binding protein. Function in phi 29 DNA-protein p3 replication

Nucleic Acids Research
G MartínM Salas

Abstract

The phage phi 29 protein p5, required in vivo in the elongation step of phi 29 DNA replication, was highly purified from Escherichia coli cells harbouring a gene 5-containing plasmid and from phi 29-infected Bacillus subtilis. The protein was characterized as the gene 5 product by amino acid analysis and NH2-terminal sequence determination. The purified protein p5 was shown to bind to single-stranded DNA and to protect it against nuclease degradation. No effect of protein p5 was observed either on the formation of the p3-dAMP initiation complex or on the rate of elongation. However, protein p5 greatly stimulated phi 29 DNA-protein p3 replication at incubation times where the replication in the absence of p5 leveled off.

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Citations

Aug 1, 1995·FEMS Microbiology Reviews·M SalasL Blanco
Feb 1, 1991·Biochimie·J C Alonso, G Lüder
Jan 1, 1994·Biochimie·M SerranoJ M Hermoso
Sep 1, 1991·Research in Microbiology·F RojoM Salas
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·L BlancoM Salas
Apr 25, 2000·Nucleic Acids Research·I GascónM Salas
Jan 20, 2004·Nucleic Acids Research·Verónica TrunigerMargarita Salas
Feb 20, 2004·Nucleic Acids Research·Wilfried J J Meijer, Margarita Salas
May 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·W J MeijerM Salas
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·J A EstebanM Salas
Apr 6, 2006·FEMS Microbiology Reviews·Christoph Weigel, Harald Seitz
Jan 18, 2005·FEMS Microbiology Reviews·Alicia BravoMargarita Salas
Aug 23, 2016·Frontiers in Molecular Biosciences·Margarita SalasMiguel de Vega
May 21, 2019·PloS One·Iván de la TorreAlicia Del Prado
Feb 5, 1999·Journal of Molecular Biology·V TrunigerM Salas

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