Characterization of the polyketide spore pigment cluster whiESa in Streptomyces aureofaciens CCM3239

Archives of Microbiology
Renata NovakovaJan Kormanec

Abstract

A spore pigment polyketide gene cluster, whiESa, was cloned from Streptomyces aureofaciens CCM3239 using a probe from the S. coelicolor A3(2) whiE gene cluster. Sequence analysis of a 4,657-bp DNA fragment revealed five open reading frames with the highest similarity to the S. coelicolor A3(2) whiE locus responsible for spore pigment biosynthesis, with conservation of the size and position of the genes. The whiESa gene cluster was disrupted by a homologous recombination in S. aureofaciens CCM3239, replacing the most important whiESaIII gene encoding ketosynthase with a thiostrepton resistance gene. The mutation affected spore pigmentation. In contrast to wild-type grey-pink spore pigmentation, the mutant produced white spores, although overall spore morphology was not affected. Transcriptional analysis of whiESa revealed two divergently oriented promoters, whiESap1 and whiESap2, upstream of the whiESaI and whiESaVIII genes, respectively. Both promoters were developmentally regulated in S. aureofaciens CCM3239. They were induced at the late stages of differentiation, during sporulation of aerial hyphae and were dependent upon early sporulation-specific sigma factor sigma(RpoZ) and putative transcription factor WhiB. The level of...Continue Reading

References

Apr 1, 1992·Gene·F Wright, M J Bibb
Feb 1, 1996·Molecular Microbiology·M HeckerU Völker
Mar 27, 2001·Methods in Molecular Biology·J Kormanec
Feb 20, 2002·Chemical Reviews·David A. Hopwood
Oct 23, 2004·DNA Sequence : the Journal of DNA Sequencing and Mapping·Renata NovakovaJan Kormanec

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Citations

Jan 9, 2007·Journal of Bacteriology·Lei WangMeifeng Tao
Dec 14, 2011·Chembiochem : a European Journal of Chemical Biology·Xiaohui YanAndreas Bechthold
Nov 15, 2012·The Journal of General and Applied Microbiology·Hisayuki KomakiNobuyuki Fujita

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