PMID: 8605305Feb 1, 1996Paper

Characterization of the potato mitochondrial transcription unit containing 'native' trnS (GCU), trnF (GAA) and trnP (UGG)

Plant Molecular Biology
C Remacle, L Maréchal-Drouard

Abstract

In order to identify the sequences promoting the expression of plant mitochondrial tRNA genes, we have characterized the trnS (GCU), trnF (GAA) and trnP (UGG) transcription unit of the potato mitochondrial genome. These three tRNA genes were shown to be co-transcribed as a 1800 nt long primary transcript. The transcription initiation site located 305 to 312 nt upstream of trnS is surrounded by a purine-rich region but does not contain the consensus motif proposed as a promoter element in dicotyledonous plants. Differential labelling of potato mitochondrial RNA with either guanylyltransferase or T4 polynucleotide kinase suggests that this site corresponds to the unique functional region responsible for the transcription of the three tRNA genes. The initiation site recently found upstream of Oenothera mitochondrial trnF does not seem to be used in potato mitochondria, although a very similar sequence is present 317 nt upstream of the corresponding potato gene. Major processing sites were identified at the 3' end of each tRNA gene. Another processing site, surrounded by a double hairpin structure, is located 498 nt downstream of trnP in stretch of 10 A residues. As judged from northern experiments, this region is close to the dete...Continue Reading

References

Jan 1, 1990·Methods in Enzymology·G J GoodallW Filipowicz
Jan 1, 1991·Molecular and Cellular Biology·R M MulliganV Walbot
Aug 11, 1986·Nucleic Acids Research·W SchusterA Brennicke
Aug 1, 1982·Archives of Biochemistry and Biophysics·M NeuburgerR Douce
Jan 1, 1993·Plant Molecular Biology·F Weber-LotfiJ M Grienenberger
Jan 1, 1995·Methods in Enzymology·L Marechal-DrouardA Dietrich
Jul 1, 1985·Plant Molecular Biology·K P IamsJ H Sinclair

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Citations

Jul 20, 2002·Journal of Molecular Biology·Michaela Hoffmann, Stefan Binder
Aug 15, 1996·Nucleic Acids Research·L Maréchal-DrouardI Small
Aug 18, 1997·Biochemical and Biophysical Research Communications·H C ChenH Wintz
Jul 1, 1996·Molecular and Cellular Biology·L Marechal-DrouardA Dietrich

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