PMID: 9163877May 1, 1997Paper

Characterization of the proliferation state in canine mammary tumors by the standardized AgNOR method with postfixation and immunohistologic detection of Ki-67 and PCNA

Veterinary Pathology
C V LöhrE Weiss

Abstract

Paraffin-embedded tissue sections of 96 surgically removed mammary tumors from female dogs were analyzed for their proliferation state using three different methods. The AgNOR method, originally developed by Ploton and coworkers in 1986, modified and standardized by the AgNOR committee, is an easy, inexpensive silver-staining procedure used to determine cell proliferation and prognosis of various tumors. Due to the standardized staining protocol of the AgNOR method and a postfixation step, results obtained were of excellent quality for image-analysis processing. The growth fraction was evaluated by counting of immunohistologically positive-stained cells for Ki-67 or proliferating-cell nuclear antigen (PCNA). The values determined were, in general, lower with Ki-67 (MIB1) than with PCNA (PC10). Nevertheless, the labeling indices of these antigens correlated significantly (P < 0.001). Though the differences of the means between the tumor groups according to the classification system of the World Health Organization were significant for all three investigated methods (P < 0.001), there was a considerable overlap between the tumor groups concerning all investigated parameters. An exploratory data analysis (multivariate analysis) as...Continue Reading

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Citations

May 14, 2008·The Journal of Dairy Research·Bibliana E DallardLuis F Calvinho
Sep 8, 2000·The Journal of Small Animal Practice·M D Perez AlenzaA I Nieto
Jul 16, 2008·Journal of Veterinary Internal Medicine·K Bundgaard-AndersenD Trerè
Jan 23, 1999·Research in Veterinary Science·M CastagnaroM Caramelli
Nov 5, 2008·Veterinary Dermatology·Monika M WelleSilvia Rüfenacht
Feb 19, 2009·Veterinary and Comparative Oncology·C EcksteinC Rohrer Bley
Dec 20, 2014·Veterinary and Comparative Oncology·L A MestrinhoM M R E Niza
Sep 20, 2002·Acta Veterinaria Hungarica·T GuvencR Haziroglu
Mar 24, 2000·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·B S PhillipsB R Madewell
Apr 19, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Seong-Kwi KangKyoung-Oh Cho
Apr 12, 2003·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Giuseppe SarliPaolo Stefano Marcato
Sep 13, 2003·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·E HandharyaniT Umemura
Jul 27, 1999·Veterinary Pathology·M KiupelD Bostock
Sep 2, 2003·Veterinary Pathology·A GröneA Zurbriggen
Mar 14, 2000·Veterinary Pathology·Y FunakoshiK Doi
Dec 15, 2010·Veterinary Pathology·R KlopfleischA D Gruber
Mar 30, 2004·Veterinary Clinical Pathology·Debora A P C ZuccariJosé Antônio Cordeiro
Dec 31, 2014·Journal of Veterinary Science·Nilgun GultikenSelim Aslan
Oct 31, 2018·Acta Veterinaria Scandinavica·Ilona KaszakPiotr Jurka

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