Characterization of the promising poly(3-hydroxybutyrate) producing halophilic bacterium Halomonas halophila

Bioresource Technology
D KuceraS Obruca

Abstract

This work explores molecular, morphological as well as biotechnological features of the highly promising polyhydroxyalkanoates (PHA) producer Halomonas halophila. Unlike many other halophiles, this bacterium does not require expensive complex media components and it is capable to accumulate high intracellular poly(3-hydroxybutyrate) (PHB) fractions up to 82% of cell dry mass. Most remarkably, regulating the concentration of NaCl apart from PHB yields influences also the polymer's molecular mass and polydispersity. The bacterium metabolizes various carbohydrates including sugars predominant in lignocelluloses and other inexpensive substrates. Therefore, the bacterium was employed for PHB production on hydrolysates of cheese whey, spent coffee grounds, sawdust and corn stover, which were hydrolyzed by HCl; required salinity of cultivation media was set up during neutralization by NaOH. The bacterium was capable to use all the tested hydrolysates as well as sugar beet molasses for PHB biosynthesis, indicating its potential for industrial PHB production.

Citations

Feb 20, 2020·Preparative Biochemistry & Biotechnology·Tatiane Aparecida GomesMichele Rigon Spier
May 31, 2019·Frontiers in Microbiology·Tiago M M M AmaroLucilla Iacumin
Apr 5, 2020·Bioengineering·Tatiana ThomasStéphane Bruzaud
Aug 31, 2019·Bioresource Technology·Iva PernicovaStanislav Obruca
Nov 23, 2018·New Biotechnology·Karolin DietrichValérie Orsat
Feb 5, 2021·Bioresource Technology·Stanislav ObrucaMartin Koller
Jun 3, 2021·Polymers·Paulina Marciniak, Justyna Możejko-Ciesielska
Jun 17, 2021·Essays in Biochemistry·Dillirani NagarajanJo-Shu Chang
Oct 5, 2021·Frontiers in Bioengineering and Biotechnology·Sonam Dubey, Sandhya Mishra

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