Characterization of the promoter region and genomic organization of GLI, a member of the Sonic hedgehog-Patched signaling pathway

Gene
C Z LiuP M Iannaccone

Abstract

GLI is the prototype for the Gli-Kruppel gene family characterized by a consensus C2-H2 zinc finger domain and is believed to function as a transcription activator in the vertebrate Sonic hedgehog-Patched signal transduction pathway. Understanding GLI gene regulation may be of importance to understanding causes of human birth defects and cancer. To begin to understand the regulation of this developmentally important gene we have cloned the human GLI gene and functionally characterized its 5' flanking region. The GLI gene is composed of 12 exons and 11 introns and in the zinc finger coding region shares a highly conserved splicing pattern with several other Gli family members in both vertebrates and C. elegans. A major transcription initiation site was identified upstream of the GLI translation start site along with three minor transcription initiation sites. The region surrounding the transcription initiation sites lacks TATA and CCAAT consensus sequences, has a high GC content, includes a CpG island, and contains several GC boxes. A 487bp segment surrounding the transcription initiation sites increased expression of a luciferase reporter gene 15-fold in Tera-1 cells and was defined as the core promoter region of human GLI. In ...Continue Reading

References

Feb 1, 1990·Molecular and Cellular Biology·K W Kinzler, B Vogelstein
Aug 1, 1988·Molecular and Cellular Biology·J M RuppertB Vogelstein
Jan 22, 1982·Nucleic Acids Research·S M Mount
Feb 1, 1993·Developmental Dynamics : an Official Publication of the American Association of Anatomists·D WalterhouseP Iannaccone
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·G Sáfrány, R P Perry
Jul 1, 1996·Journal of Medical Genetics·L G Biesecker, J M Graham
Nov 1, 1996·Nature Genetics·E RoesslerM Muenke

❮ Previous
Next ❯

Citations

Feb 5, 2009·The Journal of Biological Chemistry·Elspeth BeauchampAykut Uren
Mar 4, 1999·Environmental Health Perspectives·D O WalterhouseP M Iannaccone
Jun 23, 2000·Archives of Biochemistry and Biophysics·Y BatardD Werck-Reichhart
Dec 31, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Anu YadavBalraj Mittal
Nov 24, 2004·Biochemical and Biophysical Research Communications·Anna DahlénIoannis Panagopoulos
Aug 19, 2015·Frontiers in Cellular Neuroscience·Cedric Boeckx, Antonio Benítez-Burraco
Sep 21, 2012·Expert Opinion on Therapeutic Targets·Mehdi H ShahiJavier S Castresana
Mar 4, 2009·Molecular Cancer·Samantha M ZunichMarilyn L G Lamm
Apr 2, 2015·Journal of Orthopaedics·Aaron W JamesSarah M Dry
Jun 4, 2004·The Journal of Investigative Dermatology·Mohammed S IkramMike Philpott
Apr 12, 2002·Genesis : the Journal of Genetics and Development·Elisabeth H VillavicencioPhilip M Iannaccone
Dec 14, 2004·Oncogene·Elizabeth A BarnesDaniel J Donoghue
Dec 27, 2011·Applied Microbiology and Biotechnology·Jing XuQuan-Hong Yao
Oct 18, 2000·The Journal of Biological Chemistry·X Q Wang, J A Rothnagel
Jan 1, 2015·Human Genome Variation·Duna BarakehFowzan S Alkuraya
Apr 18, 2003·Development Genes and Evolution·Sangeeta Dhawan, K P Gopinathan
Mar 26, 2013·Molecular Cancer Research : MCR·Joon Won YoonDavid Walterhouse
May 16, 2019·DNA Repair·Robert TaylorPhilip Iannaccone
Dec 20, 2005·Genetics·Cristina E PopescuRobert W Lee

❮ Previous
Next ❯

Related Concepts

Related Feeds

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.