Characterization of the protease of a fish retrovirus, walleye dermal sarcoma virus

Journal of Virology
Sharon K Fodor, Volker M Vogt

Abstract

Three fish retroviruses infecting walleyes constitute the recently recognized genus called epsilonretrovirus. The founding member of this group, walleye dermal sarcoma virus (WDSV), induces benign skin tumors in the infected fish and replicates near 4 degrees C. While the viral genomic sequence is known, biochemical characterization of the virus has been limited to the identification of the mature structural and envelope proteins present in virions. We undertook this study to determine the cleavage sites in the WDSV Pro and Pol proteins and to characterize the viral protease (PR) in vitro. A recombinant PR was expressed in and purified from Escherichia coli as a larger fusion with additional nucleocapsid and reverse transcriptase residues flanking the PR domain. Autocleavage produced a functional, mature PR. Autocleavage as well as cleavage of peptides and of Gag protein by the mature PR occurred at a pH optimum of 7.0, higher than that of other retroviral proteases. Analysis of the cleavage sites identified a glutamine residue in the P2 position of all WDSV sites, both in Gag and in Pol. Amino acid sequence alignments of Gag-Pro-Pol from WDSV, walleye epidermal hyperplasia virus type 1, and walleye epidermal hyperplasia virus ...Continue Reading

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May 25, 2002·The Journal of General Virology·Sharon K Fodor, Volker M Vogt

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Citations

Nov 12, 2003·Journal of Virology·Donald HolzschuMichael D Lairmore
Jul 16, 2013·Virology Journal·Guney BosoNikunj V Somia
Oct 15, 2013·PloS One·Allison Ballandras-ColasAlan Engelman
May 25, 2002·The Journal of General Virology·Sharon K Fodor, Volker M Vogt
Aug 19, 2007·The Journal of General Virology·Joel RovnakSandra L Quackenbush

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