Characterization of the Protein Tyrosine Phosphatase LmPRL-1 Secreted by Leishmania major via the Exosome Pathway

Infection and Immunity
Sabine LeithererDidier Soulat

Abstract

Similar to other intracellular pathogens, Leishmania parasites are known to evade the antimicrobial effector functions of host immune cells. To date, however, only a few virulence factors have been described for Leishmania major, one of the causative agents of cutaneous leishmaniasis. Here, we have characterized the expression and function of an L. major phosphatase, which we termed LmPRL-1. This enzyme shows a strong structural similarity to the human phosphatases of regenerating liver (PRL-1, -2, and -3) that regulate the proliferation, differentiation, and motility of cells. The biochemical characterization of the L. major phosphatase revealed that the enzyme is redox sensitive. When analyzing the subcellular localization of LmPRL-1 in promastigotes, amastigotes, and infected macrophages, we found that the phosphatase was predominantly expressed and secreted by promastigotes via the exosome route. Finally, we observed that ectopic expression of LmPRL-1 in L. major led to an increased number of parasites in macrophages. From these data, we conclude that the L. major phosphatase LmPRL-1 contributes to the intracellular survival of the parasites in macrophages.

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Citations

Jan 10, 2018·Frontiers in Immunology·Didier Soulat, Christian Bogdan
May 18, 2018·The FEBS Journal·Serge HardyMichel L Tremblay
Jan 23, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Katrin PaduchChristian Bogdan
Feb 24, 2018·Frontiers in Immunology·Helena MesslingerUlrike Schleicher
Oct 27, 2018·Journal of Clinical Microbiology·Dorit WolffChristian Bogdan
Feb 19, 2019·The American Journal of Tropical Medicine and Hygiene·Michael Constantin KirchbergerChristian Bogdan
Sep 15, 2020·Frontiers in Cellular and Infection Microbiology·Joshua Seun Olajide, Jianping Cai
Jan 31, 2019·Frontiers in Immunology·Zhenyu WuXi Sun
Mar 20, 2019·Journal of Cell Science·Adela S Oliva ChávezJoao H F Pedra
Dec 22, 2020·Journal of Immunology Research·Laura Enedina Soto-SernaIngeborg Becker
Feb 19, 2021·Molecular Immunology·Izadora Volpato RossiMarcel Ivan Ramirez
Feb 18, 2020·Biochimica Et Biophysica Acta. General Subjects·Elham DolatAmeneh Sazgarnia
May 11, 2021·Frontiers in Cellular and Infection Microbiology·Anita Leocadio Freitas-MesquitaJosé Roberto Meyer-Fernandes
Jul 27, 2021·Parasitology Research·Jenny Nancy Gómez-SandovalM Magdalena Aguirre-García

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