Characterization of the ptfA gene of avian Pasteurella multocida strains by allele-specific polymerase chain reaction

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Boglárka SellyeiT Magyar

Abstract

Pasteurella multocida is the causative agent of fowl cholera in domesticated and wild birds. The disease outcome is affected by various host- and pathogen-specific determinants. Several putative virulence factors have been proposed to play a key role in this interaction, including the ptfA gene, the products of which assemble to form type 4 fimbriae on the bacterial surface. One way to understand more precisely how ptfA contributes to pathogenesis is to gather molecular features of this gene in circulating avian P. multocida strains. Therefore, molecular characterization of the ptfA gene of P. multocida strains isolated from domestic poultry was performed using the combination of nucleotide sequence analysis and a newly developed allele-specific polymerase chain reaction assay. Two major ptfA alleles were identified among 31 strains, representing various serogroups and somatic serotypes. It was noteworthy that allele specificity and case severity of a subset of strains correlated with the available gross pathology data. Therefore, the acquisition of comprehensive clinical and epidemiological data together with molecular characteristics of individual strains will help to design and implement adequate preventive and intervention ...Continue Reading

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Citations

Jul 3, 2013·Veterinary Research Communications·Sathish Bhadravati ShivachandraK N Viswas
Feb 9, 2013·Veterinary Microbiology·Zsuzsanna VargaTibor Magyar
Feb 27, 2013·Acta Veterinaria Hungarica·Boglárka SellyeiTibor Magyar
Jun 1, 2012·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Boglárka SellyeiTibor Magyar

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Methods Mentioned

BETA
genotyping
PCR

Software Mentioned

ClustalW
Lasergene package
MegAlign

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