Characterization of the rat hippocalcin gene: the 5' flanking region directs expression to the hippocampus

Neuroscience
A L GrantW Wisden

Abstract

Hippocalcin is an EF-hand [Persechini A. et al. (1989) Trends Neurosci. 12, 462-467] Ca2+ binding protein encoded by a neuron-specific gene. A detailed atlas of hippocalcin messenger RNA expression in the adult rat brain was complied using in situ hybridization. Highest levels of messenger RNA are found in the hippocampus, where messenger RNA is localized in proximal dendrites of CA pyramidal cells. Expression is also seen in other brain regions, including the neocortex, caudate-putamen, taenia tecti, claustrum, olfactory tubercle, anterior olfactory nucleus, and granule cell and glomerular layers of the olfactory bulb. The rat hippocalcin gene spans approximately 9 kb and consists of three exons, separated by introns of 6.7 and 0.25 kb. Sequence analysis of the putative proximal promoter region identified two clusters of multiple E-box sites which may regulate the cell-specific expression. Two lacZ fusion constructs carrying 0.9 and 3.4 kb of rat hippocalcin gene upstream region were used to create transgenic mice. With the 3.4 kb construct, transgene expression varied between founder mice, but was always found in the dentate gyrus and CA1-CA4 regions of the hippocampus, thus partly mimicking the expression of the endogenous g...Continue Reading

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Citations

Aug 2, 2001·Journal of Neuroscience Methods·T Wells, D A Carter
Mar 12, 1998·Brain Research. Molecular Brain Research·A L Grant, W Wisden
Oct 26, 1999·Brain Research. Molecular Brain Research·Q B TianT Suzuki
Nov 13, 1998·Current Opinion in Neurobiology·D Kuhl, P Skehel
Apr 25, 2000·Brain Research. Brain Research Protocols·E TakahashiK Yagami
Dec 15, 2000·The Biochemical Journal·R D Burgoyne, J L Weiss
Jul 4, 2003·Genome Research·Michel de ChaldéeJean-Marc Elalouf
Dec 28, 1999·Molecular and Cellular Biology·M E Massari, C Murre
Sep 1, 2004·Biochemical and Biophysical Research Communications·Alex MammenCheil Moon
Jan 1, 1997·Molecular and Cellular Neurosciences·A HerbP H Seeburg

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